Identification of a novel conserved sorting motif required for retromer-mediated endosome-to-TGN retrieval
Identification of a novel conserved sorting motif required for retromer-mediated endosome-to-TGN retrieval
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DOI：
10.1242/jcs.009654
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发表时间：
2007-07-15
影响因子：
4
通讯作者：
Seaman, Matthew N. J.
中科院分区：
文献类型：
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作者：
Seaman, Matthew N. J.
阳离子非依赖性甘露糖 6-磷酸受体 (CIMPR) 在反式高尔基体网络 (TGN) 和内体之间循环，介导溶酶体水解酶的分选。 CIMPR 的内体到 TGN 修复需要逆转录酶复合物。遗传、生化和结构数据支持这样的假设：逆转录酶可以直接结合到 CIMPR 的尾部，将 CIMPR 分类到囊泡和小管中，以便检索到 TGN。然而，目前尚未鉴定出 CIMPR 尾部已知的逆转录酶分选基序。使用携带 CIMPR 细胞质尾部的 CD8 报告蛋白，我们系统地解剖了 CIMPR 尾部，以确定一种新颖的、保守的含芳香族分选基序，该基序对于 CIMPR 的内体到 TGN 修复以及与逆转录体和网格蛋白接头 AP-1 的相互作用至关重要。
The cation-independent mannose 6-phosphate receptor (CIMPR) cycles between the trans-Golgi network ( TGN) and endosomes to mediate sorting of lysosomal hydrolases. The endosome-to-TGN retrieval of the CIMPR requires the retromer complex. Genetic, biochemical and structural data support the hypothesis that the retromer can directly bind to the tail of the CIMPR, to sort the CIMPR into vesicles and tubules for retrieval to the TGN. Presently, however, no known retromer sorting motif in the tail of the CIMPR has been identified. Using CD8-reporter proteins carrying the cytoplasmic tail of the CIMPR we have systematically dissected the CIMPR tail to identify a novel, conserved aromatic-containing sorting motif that is critical for the endosome-to-TGN retrieval of the CIMPR and for the interaction with retromer and the clathrin adaptor AP-1.