Three-dimensional, non-invasive, cross-sectional imaging of protein crystals using ultrahigh resolution optical coherence tomography.
Three-dimensional, non-invasive, cross-sectional imaging of protein crystals using ultrahigh resolution optical coherence tomography.
复制标题
DOI：
10.1364/boe.3.000735
复制
发表时间：
2012-04-01
影响因子：
3.4
通讯作者：
Matsumura H
中科院分区：
文献类型：
--
作者：
Nishizawa N;Ishida S;Hirose M;Sugiyama S;Inoue T;Mori Y;Itoh K;Matsumura H
利用低噪声、高斯型超连续谱的超高分辨率光学相干层析成像技术(UHR-OCT)，成功地实现了蛋白质晶体的微尺度、非侵入性、三维截面成像。这项技术促进了蛋白质晶体的可视化，即使是在也含有大量沉淀物的介质中也是如此。我们发现在结晶介质中包埋琼脂糖凝胶可以增强蛋白质晶体的散射信号。用UHR-OCT观察同一样品中蛋白质和盐的结晶时，显示出不同的物理特征，表明UHR-OCT一般可以区分蛋白质和盐的结晶。Uhr-OCT是一种非破坏性和快速的方法，因此应该在设计用于可视化晶体的自动化系统中使用。
Micro-scale, non-invasive, three-dimensional cross-sectional imaging of protein crystals was successfully accomplished using ultra-high resolution optical coherence tomography (UHR-OCT) with low noise, Gaussian like supercontinuum. This technique facilitated visualization of protein crystals even those in medium that also contained substantial amounts of precipitates. We found the enhancement of the scattered signal from protein crystal by inclusion of agarose gel in the crystallization medium. Crystals of a protein and a salt in the same sample when visualized by UHR-OCT showed distinct physical characteristics, suggesting that protein and salt crystals may, in general, be distinguishable by UHR-OCT. UHR-OCT is a nondestructive and rapid method, which should therefore find use in automated systems designed to visualize crystals.