Distribution of human plasma PLTP mass and activity in hypo- and hyperalphalipoproteinemia
Distribution of human plasma PLTP mass and activity in hypo- and hyperalphalipoproteinemia
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DOI：
10.1194/jlr.m100349-jlr200
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发表时间：
2002-08-01
影响因子：
6.5
通讯作者：
Hattori, H
中科院分区：
文献类型：
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作者：
Oka, T;Yamashita, S;Hattori, H
血浆磷脂转移蛋白（PLTP）在脂蛋白代谢和逆转胆固醇转运中发挥重要作用。我们最近报道，在健康受试者中，血浆 PLTP 浓度与血浆 HDL 胆固醇 (HDL-C) 呈正相关，但与 PLTP 活性无关。我们还表明，PLTP 在健康人血浆中以活性和非活性形式存在。在本研究中，我们测量了血浆 PUP 浓度和 PLTP 活性，并分析了 PLTP 在血脂正常、胆固醇酯转运蛋白 (CETP) 缺乏和低 α 脂蛋白血症 (hypo-ALP) 受试者中的分布。低 ALP 受试者的血浆 PLTP 浓度显着较低（0.7 +/- 0.4 mg/l，平均值 +/- SD，n = 9，P < 0.001），CETP 缺乏者的血浆 PLTP 浓度显着高于对照组（19.5 +/- 4.3 mg/l，n = 17，P < 0.001）（12.4 +/- 2.3 mg/l，n = 63）。相反，我们观察到对照组、低 ALP 受试者和 CETP 缺乏者之间的血浆 PLTP 活性没有显着差异（分别为 6.2 +/- 1.3、6.1 +/- 1.8 和 6.8 +/- 1.2 mumol/ml/h）。 PLTP浓度与血浆HDL-C呈正相关（r = 0.81，n = 89，P < 0.001）。通过尺寸排阻色谱分析，我们发现，与对照组相比，低 ALP 受试者的血浆中几乎不存在含有无 PLTP 活性（PLTP 失活形式）的较大 PLTP 颗粒，并且与对照组相比，在 CETP 缺乏症的血浆中积累。（jlr）这些结果表明，低 ALP 受试者、CETP 缺乏症和对照组之间血浆 PLTP 浓度的差异主要是由于 PLTP 失活形式的量差异。
Plasma phospholipid transfer protein (PLTP) plays an important role in lipoprotein metabolism and reverse cholesterol transport. We have recently reported that plasma PLTP concentration correlates positively with plasma HDL cholesterol (HDL-C) but not with PLTP activity in healthy subjects. We have also shown that PLTP exists as active and inactive forms in healthy human plasma. In the present study, we measured plasma PUP concentration and PLTP activity, and analyzed the distribution of PLTP in normolipidemic subjects (controls), cholesteryl ester transfer protein (CETP) deficiency, and hypo-alphalipoproteinemia (hypo-ALP). Plasma PLTP concentration was significantly lower (0.7 +/- 0.4 mg/l, mean +/- SD, n = 9, P < 0.001) in the hypo-ALP subjects, and significantly higher (19.5 +/- 4.3 mg/l, n = 17, P < 0.001) in CETP deficiency than in the controls (12.4 +/- 2.3 mg/l, n = 63). In contrast, we observed no significant differences in plasma PLTP activity between controls, hypo-ALP subjects, and CETP deficiency (6.2 +/- 1.3, 6.1 +/- 1.8, and 6.8 +/- 1.2 mumol/ml/h, respectively). There was a positive correlation between PLTP concentration and plasma HDL-C (r = 0.81, n = 89, P < 0.001). By size exclusion chromatography analysis, we found that the larger PLTP containing particles without PLTP activity (inactive form of PLTP) were almost absent in the plasma of hypo-ALP subjects, and accumulated in the plasma of CETP deficiency compared with those of controls.(jlr) These results indicate that the differences in plasma PLTP concentrations between hypo-ALP subjects, CETP deficiency, and controls are mainly due to the differences in the amount of the inactive form of PLTP.