Breakpoint structure reveals the unique origin of an interspecific chromosomal inversion (2La) in the Anopheles gambiae complex
Breakpoint structure reveals the unique origin of an interspecific chromosomal inversion (2La) in the Anopheles gambiae complex
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DOI：
10.1073/pnas.0509683103
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发表时间：
2006-04-18
影响因子：
11.1
通讯作者：
Besansky, NJ
中科院分区：
文献类型：
--
作者：
Sharakhov, IV;White, BJ;Besansky, NJ
旁着丝粒染色体倒位是生物进化的主要建筑师，并与按蚊中疟疾传播相关的适应性有关。负责其起源和维护的过程，仍然知之甚少，可以通过分析反转断裂点序列来阐明。本文报道了冈比亚按蚊及其近缘种的染色体倒位2La断裂点结构。冈比亚情结。标准（2L+（a））排列的远端和近端断点包含基因复制：全长基因和它们在相对末端的截短拷贝。在交替排列中没有假基因拷贝的完整基因（2La）意味着2L+（a）是衍生的，尽管对基因有损伤，但仍然是可行的，因为重复保留了基因功能。种间2La倒位的独特起源以前受到间接遗传证据的挑战，但从A.冈比亚杂岩强烈表明它们起源于单一事件。2L+（a）的衍生位置，长期以来被认为是这一医学重要类群的祖先，对A的系统发育历史和向量能力的进化具有重要意义。冈比亚情结。
Paracentric chromosomal inversions are major architects of organismal evolution and have been associated with adaptations relevant to malaria transmission in anopheline mosquitoes. The processes responsible for their origin and maintenance, still poorly understood, can be illuminated by analysis of inversion breakpoint sequences. Here, we report the breakpoint structure of chromosomal inversion 2La from the principal malaria vector Anopheles gambiae and its relatives in the A. gambiae complex. The distal and proximal breakpoints of the standard (2L+(a)) arrangement contain gene duplications: full-length genes and their truncated copies at opposite ends. Intact genes without pseudogene copies in the alternative arrangement (2La) imply that 2L+(a) is derived and was viable despite damage to genes, because duplication preserved gene function. A unique origin for the interspecific 2La inversion was challenged previously by indirect genetic evidence, but breakpoint sequences determined from members of the A. gambiae complex strongly suggest their descent from a single event. The derived position of 2L+(a), long considered ancestral in this medically important group, has significant implications for the phylogenetic history and the evolution of vectorial capacity in the A. gambiae complex.