Hierarchical Plasmonic-Fluorescent Labels for Highly Sensitive Lateral Flow Immunoassay with Flexible Dual-Modal Switching
Hierarchical Plasmonic-Fluorescent Labels for Highly Sensitive Lateral Flow Immunoassay with Flexible Dual-Modal Switching
复制标题
用于具有灵活双模态切换的高灵敏度侧流免疫测定的分层等离子体荧光标记
DOI：
10.1021/acsami.0c18667
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发表时间：
2020-12-30
影响因子：
9.5
通讯作者：
Hu, Jun
中科院分区：
文献类型：
--
作者：
Huang, Liang;Jin, Jiening;Hu, Jun
侧流免疫分析（LFIA）作为一种重要的床旁（POC）检测平台，已被广泛用于病原体感染和疾病生物标志物的快速、现场和简便诊断。开发具有放大信号和互补检测模式的新型结构化LFIA光学标记有利于灵敏和灵活的POC诊断。在这里，通过基于亲和模板和疏水性含金属纳米单元的分层顺序组装策略制造具有比色和荧光读数的双峰标记。高质量的比色和荧光纳米粒子被密集地掺入到胶体支持物中，并被限制在分离的区域中，而不会相互干扰。金纳米粒子和量子点的分级集成具有高负载密度和良好的光学保存性，实现了从单个纳米粒子的组装体的双重读出和放大信号。“一体化”光学标记允许在与抗体表面缀合后比色和荧光检测半胱氨酸蛋白酶抑制剂C（Cys C）。LFIA条分别通过肉眼和便携式设备显示Cys C的目视检查和定量检测的非干扰双重信号。比色法和荧光法的检测限分别为0.61和0.24 ng/mL。新型LFIA平台具有灵敏、特异和可重复的生物标志物POC检测，检测模式灵活，可用于临床诊断。
Lateral flow immunoassay (LFIA), as a prominent point-of-care (POC) test platform, has been extensively adopted for rapid, on-site, and facile diagnosis of pathogen infections and disease biomarkers. Exploring novel structured optical labels of LFIA with amplified signal and complementary detection modes favors the sensitive and flexible POC diagnosis. Here, bimodal labels with both colorimetric and fluorescent readout were fabricated via a layered sequential assembly strategy based on affinity templates and hydrophobic metal-containing nanounits. High-quality colorimetric and fluorescent nanoparticles were densely incorporated into the colloidal supports and confined in separated regions, without interfering with each other. The hierarchical integration of gold nanoparticles and quantum dots with high loading density and good optical preservation realized dual readout and amplified signals from the assemblies of individual single nanoparticles. The "all-in-one" optical labels allowed both colorimetric and fluorescent detection of cystatin C (Cys C) after surface conjugation with antibodies. The LFIA strips revealed noninterfering dual signals for both visual inspection and quantitative detection of Cys C via the naked eye and portable devices, respectively. The limits of detection by colorimetric and fluorescent modes were 0.61 and 0.24 ng mL(-1), respectively. The novel LFIA platform demonstrated sensitive, specific, and reproducible POC testing of biomarkers with flexible detection modes and was reliable for clinical diagnosis.