Innate and acquired immunity to herpes simplex virus type 1
Innate and acquired immunity to herpes simplex virus type 1
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DOI：
10.1006/viro.1997.8738
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发表时间：
1997-09-29
期刊：
影响因子：
3.7
通讯作者：
Carr, DJJ
中科院分区：
文献类型：
--
作者：
Halford, WP;Veress, LA;Carr, DJJ
眼部感染前2-5天接种热灭活的1型单纯疱疹病毒（HSV-1）可降低三叉神经节（TG）潜伏性HSV-1感染的发生频率；这种抗性的诱导与甘油三酯中ifn - γ mRNA表达的降低相一致。不相关抗原免疫无保护作用。在某种程度上，这种对神经系统入侵的抵抗与HSV-1血清抗体的出现有关。免疫减少了病毒在眼睛和三叉神经节中的复制，并防止HSV-1扩散到小脑。通过中和抗体对ifn - α / β和ifn - γ的斑块减少来检测免疫小鼠囊泡感染后4天的ifn - γ。免疫期间注射ifn - α / β和ifn - γ抗体（Ab）不影响存活。抗干扰素γ处理小鼠血清中干扰素水平显著降低。抗ifn - α / β - Ab治疗可通过小鼠TG中潜伏期相关转录物的表达来确定病毒复制的升高。同样，抗ifn - α / β处理小鼠TG中CD8、IL-12 （p40）和TNF-LU mRNA水平显著升高。TG外植体培养表明，感染后7天，抗ifn - α / β处理小鼠的TG中病毒载量明显高于对照组小鼠。结果表明，感染前2-5天暴露于病毒抗原是1型单纯疱疹病毒向神经系统传播程度的重要决定因素。此外，数据表明抗体反应和ifn - α / β都在限制感染从外周组织向中枢神经系统的进展中发挥作用。(C) 1997学术出版社。
Immunization with heat-inactivated herpes simplex virus type 1 (HSV-I) 2-5 days before ocular infection reduced the frequency of establishment of latent HSV-1 infection in the trigeminal ganglion (TG); this induction of resistence coincided with reduced expression of IFN-gamma mRNA in the TG. Immunization with unrelated antigens was not protective. In part, this resistance to nervous system invasion correlated with the appearance of serum antibody to HSV-1. Immunization reduced viral replication in the eye and trigeminal ganglion, and prevented HSV-1 spread to the cerebellum. IFN-gamma was detected in immunized mice 4 days postocular infection as determined by plaque reduction using neutralizing Ab to IFN-alpha/beta and IFN-gamma. Injection of antibody (Ab) to IFN-alpha/beta and IFN-gamma administered al the time of immunization did not affect survival. Anti-IFN-gamma-treated mice had significantly reduced levels of IFN in their serum. Treatment with anti-IFN-alpha/beta Ab resulted in an elevation in viral replication as determined by the expression or latency associated transcripts in the TG of mice. Likewise, there was a significant increase in the CD8, IL-12 (p40), and TNF-LU mRNA levels in the TG of the anti-IFN-alpha/beta-treated mice. TG explant cultures demonstrated that viral load was significantly increased in the TG of anti-IFN-alpha/beta-treated mice relative to TG of control mice 7 days after infection. The results suggest that exposure to viral antigens 2-5 days before infection is an important determinant of the extent of HSV-1 spread to the nervous system. Moreover, the data suggest that both an antibody response and IFN-alpha/beta play a role in limiting the progress of infection from the peripheral tissues to the central nervous system. (C) 1997 Academic Press.