Stimulation of medial amygdala GABA neurons with kinetically different channelrhodopsins yields opposite behavioral outcomes
Stimulation of medial amygdala GABA neurons with kinetically different channelrhodopsins yields opposite behavioral outcomes
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DOI：
10.1016/j.celrep.2022.110850
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发表时间：
2022-05-24
期刊：
影响因子：
8.8
通讯作者：
Kochubey, Olexiy
中科院分区：
文献类型：
--
作者：
Baleisyte, Aiste;Schneggenburger, Ralf;Kochubey, Olexiy
内侧杏仁核 (MeA) 接收有关同种动物的信息素信息，在社会行为中具有至关重要的功能。先前的一项研究表明，用通道视紫红质-2(H)(134R) (ChR2) 激活后背 MeA (MeApd) 中的 GABA 神经元会刺激雄性间的攻击行为。当使用更快的通道视紫红质 (H134R)(,)(E123T) (ChETA) 进行这些实验时，我们发现了相反的行为结果。两种通道视紫红质变体之间的系统比较表明，ChETA 光遗传学激活 MeApd GABA 神经元可抑制攻击性，而 ChR2 下的激活会增加攻击性。尽管这种矛盾差异的机制尚不清楚，但我们观察到，与 ChETA 相比，用 ChR2 激活 MeApd GABA 神经元会导致更大的平台去极化、更小的动作电位和更大的局部抑制。因此，用于体内光遗传学实验的视紫红质通道蛋白变体可以从根本上影响行为结果。未来的工作应继续研究 MeApd GABA 神经元的特定亚群在攻击性控制中的作用。
The medial amygdala (MeA) receives pheromone information about conspecifics and has crucial functions in social behaviors. A previous study showed that activation of GABA neurons in the postero-dorsal MeA (MeApd) with channelrhodopsin-2(H)(134R) (ChR2) stimulates inter-male aggression. When performing these experiments using the faster channelrhodopsin(H134R)(,)(E123T) (ChETA), we find the opposite behavioral outcome. A systematic comparison between the two channelrhodopsin variants reveals that optogenetic activation of MeApd GABA neurons with ChETA suppresses aggression, whereas activation under ChR2 increases aggression. Although the mechanism for this paradoxical difference is not understood, we observe that activation of MeApd GABA neurons with ChR2 causes larger plateau depolarizations, smaller action potentials, and larger local inhibition than with ChETA. Thus, the channelrhodopsin variant used for in vivo optogenetic experiments can radically influence the behavioral outcome. Future work should continue to study the role of specific sub-populations of MeApd GABA neurons in aggression control.