OCCURRENCE OF VIP AND PEPTIDE HM IN HUMAN-PANCREAS AND THEIR INFLUENCE ON PANCREATIC ENDOCRINE SECRETION IN MAN
OCCURRENCE OF VIP AND PEPTIDE HM IN HUMAN-PANCREAS AND THEIR INFLUENCE ON PANCREATIC ENDOCRINE SECRETION IN MAN
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DOI：
10.1016/0167-0115(87)90050-4
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发表时间：
1987-07-01
影响因子：
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通讯作者：
LUNDBERG, JM
中科院分区：
文献类型：
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作者：
FAHRENKRUG, J;PEDERSEN, JH;LUNDBERG, JM
通过免疫组织化学方法，发现VIP和肽HI/肽HM（PHI/PHM）样免疫反应性发生在人胰腺的自主神经元。抗VIP和PHI/PHM的抗血清与局部神经节的神经元细胞反应，这些神经节也含有PHI/PHM和VIP免疫反应纤维丛。VIP-和PHI/PHM-阳性纤维也被看到接近朗格汉斯''胰岛。此外，PHI/PHM-而不是VIP样免疫反应性观察内分泌细胞往往位于周围的胰岛。这些PHI/PHM阳性细胞的性质仍有待确定。以300和900 pmol/kg ×的恒定速率静脉输注VIP。在6名健康志愿者中，持续30分钟，导致102 ± 1.2h的平台值。26和291 .+-. 25 pM。这些高于生理条件下循环中发现的VIP水平的VIP剂量依赖性地刺激胰岛素、C肽和胰高血糖素的分泌。相反，延长（60分钟）PHM输注的剂量导致血浆水平高达1340 ± 0.001。405 pM对胰腺激素分泌无影响。这些结果表明，VIP是一个可能的神经递质在控制内分泌胰腺分泌，而PHM有一个不太突出的作用，如果有的话。
By immunohistochemistry it was found that VIP- and peptide HI/peptide HM (PHI/PHM)-like immunoreactivity occurred in autonomic neurons in the human pancreas. Antisera against both VIP and PHI/PHM reacted with neuronal cells in local ganglia and these ganglia also contained PHI/PHM-and VIP-immunoreactive fibre plexuses. VIP- and PHI/PHM-positive fibres were also seen close to the Langerhans'' islets. In addition, PHI/PHM- but not VIP-like immunoreactivity was observed in the endocrine cells often located in the periphery of the islets. The nature of these PHI/PHM-positive cells remains to be established. I.v. infusion of VIP at constant rates of 300 and 900 pmol/kg .times. h for 30 min in 6 healthy volunteers resulted in plateau values of 102 .+-. 26 and 291 .+-. 25 pM, respectively. These levels of VIP which are above those found in the circulation under physiological conditions stimulated secretion of insulin, C-peptide and pancreatic glucagon dose-dependently. On the contrary prolonged (60 min) infusion of PHM in doses resulting in plasma levels up to 1340 .+-. 405 pM had no effect on pancreatic hormone secretion. These findings suggest that VIP is a likely neurotransmitter in the control of endocrine pancreatic secretion while PHM has a less prominent role, if any.