PARATHYROID-HORMONE STIMULATES THE PROLIFERATION OF CELLS DERIVED FROM HUMAN-BONE
PARATHYROID-HORMONE STIMULATES THE PROLIFERATION OF CELLS DERIVED FROM HUMAN-BONE
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DOI：
10.1210/endo-118-6-2445
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发表时间：
1986-06-01
期刊：
影响因子：
4.8
通讯作者：
RUSSELL, RGG
中科院分区：
文献类型：
--
作者：
MACDONALD, BR;GALLAGHER, JA;RUSSELL, RGG
尽管在体外对骨形成有急性抑制作用，但PTH已被证明在体内对骨有合成代谢作用，并刺激成骨细胞系和器官培养中的细胞增殖。我们研究了甲状旁腺激素对人类小梁骨细胞的影响，并将这些影响与对人类皮肤成纤维细胞的影响进行了比较。人骨细胞具有合成I型胶原蛋白和骨钙素的能力，并且对1,25-二羟基维生素D3有反应，骨钙素和碱性磷酸酶的合成增加。在低和高细胞密度下，甲状旁腺激素都能刺激腺苷化酶环化酶的活性。然而，相同浓度的激素只有在高细胞密度下培养时才会刺激这些细胞的增殖。PTH的作用是骨细胞特异性的，在相同供体和相同条件下培养的人皮肤成纤维细胞中未检测到PTH的增殖作用。甲状旁腺激素对人骨细胞DNA合成的影响可能在对甲状旁腺激素产生长期合成代谢反应中是重要的。
Despite its acute inhibitory effect on bone formation in vitro, PTH has been shown to have an anabolic effect on bone in vivo and to stimulate cell proliferation in osteoblastic cell lines and organ cultures. We have examined the effects of PTH on cells derived from human trabecular bone and compared these effects with those on human skin fibroblasts. Human bone cells have the capacity to synthesize type I collagen and osteocalcin, and to respond to 1,25-dihydroxyvitamin D3 with an increase in the synthesis of osteocalcin and alkaline phosphatase. PTH stimulated adenylase cyclase activity at both low and high cell density. However, the same concentrations of hormone stimulated the proliferation of these cells only when they were cultured at a high cell density. The effect of PTH was bone cell specific in that no proliferative effect of PTH was detected in cultures of human skin fibroblasts obtained from the same donor and cultured under the same conditions. The effect of PTH on DNA synthesis by human bone cells may be important in the generation of a long term anabolic response to PTH.