IDENTIFICATION AND CHARACTERIZATION OF A HERPES-SIMPLEX VIRUS GENE-PRODUCT REQUIRED FOR ENCAPSIDATION OF VIRUS-DNA
IDENTIFICATION AND CHARACTERIZATION OF A HERPES-SIMPLEX VIRUS GENE-PRODUCT REQUIRED FOR ENCAPSIDATION OF VIRUS-DNA
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DOI：
10.1128/jvi.45.3.1056-1064.1983
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发表时间：
1983-01-01
影响因子：
5.4
通讯作者：
RIXON, FJ
中科院分区：
文献类型：
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作者：
PRESTON, VG;COATES, JAV;RIXON, FJ
单纯疱疹病毒1型突变体17tsVP1201在晚期病毒多肽中具有温度敏感性加工缺陷。用单克隆抗体进行的免疫沉淀研究表明，突变病毒感染的人胎肺成纤维细胞中的异常多肽是称为p40的核衣壳多肽。由于回复突变体（TS+生长）通常在限制突变体的条件下加工多肽，因此加工事件对病毒复制必不可少。在非允许温度下生长的突变病毒感染的细胞的EM分析显示，细胞核含有具有某些内部结构的空核衣壳的大聚集体。因此，尽管突变体在非允许温度下合成病毒DNA，但DNA没有包装成核衣壳。当突变病毒感染的细胞从39 ° C移动到10 ° C时，到31度。在放线菌酮存在下，多肽p40被加工成较低MW形式，并且在细胞核中检测到完整的核衣壳。在非允许温度下，突变体的异常多肽在用17tsVP 1201和回复突变体混合感染的细胞中不被加工，这表明突变体的缺陷在于编码p40的基因而不是加工酶的基因。
A mutant of herpes simplex virus type 1, 17tsVP1201, has a temperature-sensitive processing defect in a late virus polypeptide. Immunoprecipitation studies with monoclonal antibodies showed that the aberrant polypeptide in mutant virus-infected human fetal lung fibroblast cells was the nucleocapsid polypeptide known as p40. Since a revertant, TS+ for growth, processed the polypeptide normally under conditions restrictive for the mutant, the processing event must be essential for virus replication. EM analysis of mutant virus-infected cells grown at the nonpermissive temperature revealed that the nuclei contained large aggregations of empty nucleocapsids possessing some internal structure. Thus, although the mutant synthesized virus DNA at the nonpermissive temperature, the DNA was not packaged into nucleocapsids. When mutant virus-infected cells were shifted from 39.degree. to 31.degree. C in the presence of cycloheximide, the polypeptide p40 was processed to lower MW forms, and full nucleocapsids were detected in the cell nuclei. The aberrant polypeptide of the mutant was not processed in cells mixedly infected with 17tsVP1201 and a revertant at the nonpermissive temperature, suggesting that the defect of the mutant was in the gene encoding p40 rather than in a gene of a processing enzyme.