DETERMINANTS OF ANTIBODY-RESPONSE AFTER RECOMBINANT GP160 BOOSTING IN VACCINIA-NAIVE VOLUNTEERS PRIMED WITH GP160-RECOMBINANT VACCINIA VIRUS
DETERMINANTS OF ANTIBODY-RESPONSE AFTER RECOMBINANT GP160 BOOSTING IN VACCINIA-NAIVE VOLUNTEERS PRIMED WITH GP160-RECOMBINANT VACCINIA VIRUS
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DOI：
10.1093/infdis/170.4.782
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发表时间：
1994-10-01
影响因子：
6.4
通讯作者：
SMITH, GE
中科院分区：
文献类型：
--
作者：
GRAHAM, BS;GORSE, GJ;SMITH, GE
用活的重组载体引发，随后进行亚单位加强是用于人类免疫缺陷病毒（HIV）免疫的有希望的策略。用gp 160-重组痘苗病毒（HIVAC-1 e）对29名未接种过疫苗的志愿者进行初免，并用重组（r）gp 160进行加强免疫，以确定与加强免疫后抗体应答的幅度和特异性相关的因素。接种和加强之间的较长间隔，两次接种HIVAC-1 e后发生病变形成，免疫印迹可检测抗体的gp 160接种后与较高的中和抗体滴度和融合抑制活性后加强。HIVAC-1 e致敏的疫苗接种者比单独用rgp 160免疫的疫苗接种者更可能具有针对gp 120的V3和CD 4结合区的抗体，而不太可能具有针对恒定区2和3的抗体。用HIVAC-1 e致敏志愿者是rgp 160加强诱导的表位特异性和功能性抗体应答幅度的关键决定因素。
Priming with a live recombinant vector followed by subunit boosting is a promising strategy for human immunodeficiency virus (HIV) immunization. Twenty-nine vaccinia-naive volunteers were primed with gp160-recombinant vaccinia virus (HIVAC-1e) and boosted with recombinant (r) gp160 to define factors associated with the magnitude and specificity of antibody response after booster immunization. A longer interval between inoculation and boost, two inoculations of HIVAC-1e with lesion formation occurring after the first, and Western blot-detectable antibody to gp160 after inoculation were significantly associated with higher neutralizing antibody titers and fusion-inhibiting activity after boosting. HIVAC-1e-primed vaccinees were more likely to have antibody to V3- and CD4-binding regions of gp120 and less likely to have antibody to constant regions 2 and 3 than vaccinees immunized with rgp160 alone. Priming volunteers with HIVAC-1e was a key determinant of the epitope specificity and magnitude of functional antibody responses induced by rgp160 boosting.