Chemokines and cytokines profile in whole saliva of patients with periodontitis
Chemokines and cytokines profile in whole saliva of patients with periodontitis
复制标题
DOI：
10.1016/j.cyto.2020.155197
复制
发表时间：
2020-11-01
期刊：
影响因子：
3.8
通讯作者：
Alves Mayer, Marcia Pinto
中科院分区：
文献类型：
--
作者：
Kawamoto, Dione;Lucas Amado, Pamela Pontes;Alves Mayer, Marcia Pinto
临床特征表明牙周炎形式之间的免疫反应的差异，尽管大量的细胞因子和趋化因子仍有待评估。唾液是介质的可用来源，其分析将是有价值的，以了解病理生理差异。本研究的目的是分析具有中度[B级; GB]或快速进展率并具有局部切牙-磨牙模式[C级; GC/IMP]的重度牙周炎（III期）个体的全唾液中的趋化因子/细胞因子谱。对每个牙周炎组进行病例对照研究。对GB（n = 9）和GC/IMP（n = 7）患者及其健康对照（C-GB，n = 9和C-GC，n = 7）进行了评价。非刺激的唾液样品通过多重测定评估总共40种细胞因子、C-C和C-X-C基序趋化因子。当与其对照相比时，GC/IMP组呈现出更高水平的CCL 17和CCL 27（p = 0.04，FDR > 0.05），以及更低水平的CCL 2（p = 0.04，FDR > 0.05）和CCL 25（p = 0.006，FDR < 0.05）。GB患者的IL-6、IL-1 β水平较高，（p = 0.04，FDR > 0.05），以及升高的促炎性细胞因子（TNF-α）。（TNF-α、IL-1 β、INF-γ、IL-6、IL-16）：抗炎01，FDR < 0.05）[通过Mann-Whitney检验的p值，和通过Benjamini-Hochburg校正的假发现率（FDR）]。CCL-趋化因子和细胞因子分别促成GC/C-GC和GB/C-GB之间的差异（p < 0.05，PERMANOVA检验）。这些初步数据显示，每种牙周炎表型与其相关对照相比在唾液中呈现不同的免疫特征，表明GB和GC/IMP的发病机制存在差异。
Clinical features suggest differences in immune response among periodontitis forms, albeit a large number of cytokines and chemokines remain to be evaluated. The saliva is an available source of mediators and its analysis would be valuable in order to understand pathophysiological differences. The objective of this study was analyze chemokines/cytokines profile in whole saliva of individuals with severe periodontitis (Stage III) presenting moderate [Grade B; GB] or rapid progression rate with a localized incisor-molar pattern [Grade C; GC/IMP]. A case-control study was designed for each periodontitis group. GB (n = 9) and GC/IMP (n = 7) patients and their healthy controls (C-GB, n = 9 and C-GC, n = 7) were evaluated. Non-stimulated saliva samples were assessed by a multiplex assay for a total of 40 cytokines, C-C and C-X-C motif chemokines. GC/IMP group presented higher levels of CCL17 and CCL27 (p = 0.04, FDR > 0.05), and lower levels of CCL2 (p = 0.04, FDR > 0.05) and CCL25 (p = 0.006, FDR < 0.05) when compared to its control. GB patients had higher levels of IL-6, IL-1 beta (p = 0.04, FDR > 0.05), and elevated pro-inflammatory (TNF-alpha,IL-1 beta,INF-gamma,IL-6, IL-16): anti-inflammatory (IL-2, IL-4, IL-10) ratio (p = 0.01, FDR < 0.05) compared to its control [p-values by Mann-Whitney test, and False Discovery Rate (FDR) by Benjamini-Hochburg corrections]. CCL-chemokines and cytokines contributed to differences between GC/C-GC and GB/C-GB, respectively (p < 0.05, PERMANOVA test). These preliminary data revealed that each periodontitis phenotype presented distinct immune profiles differentially expressed in saliva compared to their related controls, suggesting differences in the etiopathogenesis of GB and GC/IMP.