COMPARISON OF 5 METHODS, INCLUDING THE PDM EPSILOMETER TEST (E-TEST), FOR ANTIMICROBIAL SUSCEPTIBILITY TESTING OF PSEUDOMONAS-AERUGINOSA
COMPARISON OF 5 METHODS, INCLUDING THE PDM EPSILOMETER TEST (E-TEST), FOR ANTIMICROBIAL SUSCEPTIBILITY TESTING OF PSEUDOMONAS-AERUGINOSA
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DOI：
10.1128/jcm.30.10.2709-2713.1992
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发表时间：
1992-10-01
影响因子：
9.4
通讯作者：
ANDREW, JH
中科院分区：
文献类型：
--
作者：
JOYCE, LF;DOWNES, J;ANDREW, JH
采用美国国家临床实验室标准委员会（NCCLS）的肉汤微量稀释法、琼脂稀释法和琼脂盘扩散法检测100株铜绿假单胞菌临床分离株对6种抗假单胞菌抗生素的敏感性；Vitek自动微生物系统方法（Vitek Systems, Hazelwood, Mo.）；以及PDM Epsilometer试验（E试验）（AB Biodisk, Solna, Sweden）。E试验结果显示与琼脂稀释结果具有良好的相关性，在1倍稀释范围内，E试验和参考琼脂稀释mic之间的一致性超过90%。E检验结果与参考琼脂盘扩散法的结果也显示出良好的相关性，所有抗生素的分类完全吻合90 ~ 99%，基本吻合100%（基本吻合是忽略微小差异后得出的吻合）。以微量肉汤稀释法为参比法，与E检验和微量肉汤稀释法的分类比较，庆大霉素的完全一致性只有59%，有28个小差异，13个非常大差异。庆大霉素的E试验和肉汤法的结果存在一些差异，肉汤微量稀释法和Vitek法的mic高于E试验和其他琼脂法。最新的NCCLS肉汤稀释试验指南降低了阳离子补充的推荐水平，这可能会增强肉汤和琼脂上氨基糖苷和铜绿假单胞菌结果之间的一致性。我们发现E试验为apr培养基上的MIC测定提供了一种简单、省力、准确的方法。
The antimicrobial susceptibilities of 100 clinical isolates of Pseudomonas aeruginosa to six antipseudomonal antibiotics were tested by five methods: the National Committee for Clinical Laboratory Standards (NCCLS) methods for broth microdilution, agar dilution, and agar disk diffusion; the Vitek Automicrobic System method (Vitek Systems, Hazelwood, Mo.); and the PDM Epsilometer test (E test) (AB Biodisk, Solna, Sweden). The E test results showed excellent correlation with agar dilution results, with over 90% agreement within 1 doubling dilution between the E test and reference agar dilution MICs for all antimicrobial agents tested. The E test results also showed good correlation with the results from the reference agar disk diffusion method, with 90 to 99% complete agreement and 100% essential agreement on categories for all antibiotics tested (essential agreement is the agreement obtained when minor discrepancies are ignored). Comparison of categories with the E test and broth microdilution methods, using the broth microdilution method as the reference method, gave only 59% complete agreement for gentamicin, with 28 minor discrepancies and 13 very major discrepancies. Some discrepancies were observed between results from the E test and broth methods for gentamicin, with the broth microdilution and Vitek methods giving higher MICs than the E test and other methods using agar. The most recent NCCLS guidelines for broth dilution testing have reduced the recommended levels of cation supplementation, which may enhance future agreement between results for the aminoglycosides and P. aeruginosa on broth and on agar. We found that the E test offers a simple, labor-efficient, and accurate method for MIC determination on an apr medium.