The association between bacterial colonization and inflammatory pattern in Chinese chronic rhinosinusitis patients with nasal polyps
The association between bacterial colonization and inflammatory pattern in Chinese chronic rhinosinusitis patients with nasal polyps
复制标题
中国慢性鼻窦炎伴鼻息肉患者细菌定植与炎症模式的关系
DOI：
10.1111/j.1398-9995.2011.02637.x
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发表时间：
2011-10-01
期刊：
影响因子：
12.4
通讯作者：
Bachert, C.
中科院分区：
文献类型：
--
作者：
Ba, L.;Zhang, N.;Bachert, C.
背景：慢性鼻窦炎伴鼻息肉（CRSwNP）可根据粘膜炎症模式进行细分。在中国大陆，除了白细胞介素（IL）-5阳性和IL-17阳性息肉外，大量IL-5/IL-17/干扰素-γ（IFN γ）阴性鼻息肉（被称为关键性大肠杆菌阴性（KCN）息肉）。目的：为了进一步研究KCN息肉，并评估KCN与IL-5的细菌定植和粘膜炎症模式之间的关系，方法：鼻息肉或鼻甲组织取自89例CRSwNP患者和36例正常对照者。处理未接触SEB和接触SEB后的样本，通过免疫测定法评估促炎细胞因子和介质。手术前，鼻拭子取自每个病人的微生物evaluation.Results：总体而言，80%的息肉组织不表达IL-5，其中约70%（49/71）的KCN。关键的嗜酸性粒细胞阴性鼻息肉的特征在于促进嗜酸性粒细胞炎症的介质（髓过氧化物酶（MPO）、IL-1 β、IL-6和IL-8）的合成，而IL-5阳性鼻息肉的特征在于促进嗜酸性粒细胞炎症的介质（IL-5、ECP、总IgE和SE-IgE）的合成。与对照组相比，关键的大肠杆菌阴性鼻息肉与更大的革兰氏阴性细菌负荷相关，而IL-5阳性鼻息肉与更大的革兰氏阳性细菌定植与对照组和KCN polyps.Conclusion：我们的研究结果表明，细菌定植CRSwNP患者的鼻息肉可能会影响或由IL-5的存在/不存在决定。
Background: Chronic rhinosinusitis with nasal polyps (CRSwNP) can be subdivided according to the mucosal inflammatory patterns. In mainland China, apart from interleukin (IL)-5-positive and IL-17-positive polyps, a large group of patients with IL-5/IL-17/interferon-gamma (IFN gamma)-negative nasal polyps (referred to as key cytokine-negative (KCN) polyps) can be found.Objective: To further study the KCN polyps and evaluate the associations between bacterial colonization and mucosal inflammatory pattern in KCN vs IL-5-positive nasal polyps.Methods: Nasal polyp or nasal turbinate tissue was obtained from 89 Chinese CRSwNP patients and 36 nonatopic control subjects during surgery. Samples without and after SEB exposure were processed for the assessment of pro-inflammatory cytokines and mediators by immunoassay. Prior to surgery, nasal swabs were taken from each patient for microbiological evaluation.Results: Overall, 80% polyp tissue did not express IL-5, with about 70% (49/71) of these being KCN. Key cytokine-negative nasal polyps were characterized by the synthesis of mediators promoting neutrophilic inflammation (myeloperoxidase (MPO), IL-1 beta, IL-6 and IL-8), whereas IL-5-positive nasal polyps were characterized by the synthesis of mediators promoting eosinophilic inflammation (IL-5, ECP, total IgE and SE-IgE). Key cytokine-negative nasal polyps were associated with greater Gram-negative bacterial load compared with controls, while IL-5-positive nasal polyps were associated with greater Gram-positive bacterial colonization vs controls and KCN polyps.Conclusion: Our findings suggest that the bacteria colonizing nasal polyps of CRSwNP patients may impact on or be determined by the presence/absence of IL-5.