Functionalization of acyclic xenonucleic acid with modified nucleobases
Functionalization of acyclic xenonucleic acid with modified nucleobases
复制标题
用修饰的核碱基对无环异核酸进行功能化
DOI：
10.1038/s41428-023-00776-7
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发表时间：
2023
期刊：
影响因子：
2.8
通讯作者：
Asanuma Hiroyuki
中科院分区：
文献类型：
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作者：
Murayama Keiji;Yamano Yuuhei;Asanuma Hiroyuki
异种核酸(XNAs)由人工支架和天然碱基组成，具有独特的杂交性能，取决于支架的结构。在这里，我们用非天然碱基功能化了无环XNA丝氨醇核酸(SNA)。用5-二苯乙炔基尿嘧啶残基功能化的线性SNA探针在没有靶RNA的情况下表现出微弱的绿黄色准分子发射，而在靶RNA存在的情况下表现出明亮的蓝绿色单体发射。探针杂交速度快，能够定量测定RNA，并能区分单碱基错配。我们还设计了一种含有两个8-戊二烯基腺嘌呤(PVA)残基的光响应性SNA。蓝光(455 nm)照射可引起PVA链内[2RNA 2]光环加成反应，导致 + /RNA二聚体解离，而紫外光(340 nm)照射可引起PVA光二聚体回转和SNARNA二聚体重构。用8-萘基乙烯基腺嘌呤与聚乙烯醇结合，在465 nm、405 nm、340 nm和30 0 nm的光照下，对SNA(NVA-NVA)/RNA和SNA(PVA-NVA)/RNA二聚体的形成进行了正交控制。因此，碱基修饰进一步扩大了无环XNA在生物纳米技术中的应用。
Xenonucleic acids (XNAs), which are composed of artificial scaffolds and natural nucleobases, have unique hybridization properties that depend on the scaffold structure. Here, we functionalized the acyclic XNA serinol nucleic acid (SNA) with nonnatural nucleobases. A linear SNA probe functionalized with 5-perylenylethynyl uracil residues showed weak greenish-yellow excimer emission in the absence of target RNA and bright cyan-green monomer emission in the presence of target RNA. Probe hybridization was rapid and enabled the quantitative measurement of RNA with discrimination of single-base mismatches. We also designed a photoresponsive SNA with two 8-pyrenylvinyl adenine (PVA) residues. Irradiation with blue (455 nm) light caused [2 + 2] photocycloaddition between intrastrandPVAs, resulting in the dissociation of the SNA/RNA duplex, whereas irradiation with ultraviolet (340 nm) light induced cycloreversion of thePVA photodimer and SNA/RNA duplex reformation. Using a combination of 8-naphthylvinyl adenine (NVA) andPVA and irradiation with 465 nm, 405 nm, 340 nm, and 300 nm light, orthogonal control of the formation of SNA(NVA-NVA)/RNA and SNA(PVA-NVA)/RNA duplexes was demonstrated. Thus, nucleobase modifications further expand the utility of acyclic XNA in bionanotechnology.