New blood vessel formation and expression of VEGF receptors after implantation of platelet growth factor-enriched biodegradable nanocrystalline hydroxyapatite
New blood vessel formation and expression of VEGF receptors after implantation of platelet growth factor-enriched biodegradable nanocrystalline hydroxyapatite
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DOI：
10.1080/08977190500126306
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发表时间：
2005-06-01
期刊：
影响因子：
1.8
通讯作者：
Schnettler, R
中科院分区：
文献类型：
--
作者：
Kilian, O;Alt, V;Schnettler, R
血管内皮生长因子（VEGF）在成骨细胞和内皮细胞的相互作用中起关键作用，因此是骨替代物骨整合的重要因素。本研究旨在观察血小板生长因子（PLF）对纳米晶羟基磷灰石（HA）骨缺损新生血管形成及VEGF受体表达的影响。常规组织学检查、VEGF及其受体mRNA的RT-PCR、Western blot分析、免疫组化染色和新生血管的定量评估。富含血小板生长因子的HA（HA/PLF+）导致VEGF-R1合成的上调，与没有PLF的HA（HA/PLF-）填充缺陷相比，具有较高发芽活性的新形成的血管的数量略微增加。这些观察结果最有可能归因于血小板因子TGF-β对成骨细胞中VEGF表达的刺激作用。
Vascular endothelial growth factor (VEGF) plays a key role for the interaction of osteoblasts and endothelial cells and, therefore, is an important factor for the osteointegration of bone substitutes. The aim of the current work was to study the effects of platelet growth factors (PLF) on new blood vessel formation and VEGF-receptors expression pattern in bone defects filled with nanocrystalline hydroxyapatite (HA) paste in miniature-pigs. Conventional histology, RT-PCR for VEGF and receptors mRNA, Western blot analysis, immunohistochemical staining and quantitative assessment of newly formed vessels was performed. HA enriched with platelet growth factor (HA/PLF+) led to an up-regulation of VEGF-R1 synthesis, a slightly enhanced number of newly formed vessels with higher sprouting activity compared with HA without PLF (HA/PLF-) filling defects. These observation are most likely attributable to a stimulating effect of TGF-beta from the platelet factor on VEGF expression in osteoblasts.