Recombinant Protein Production in Plants: A Brief Overview of Strengths and Challenges.
Recombinant Protein Production in Plants: A Brief Overview of Strengths and Challenges.
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DOI：
10.1007/978-1-0716-2241-4_1
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发表时间：
2022-01-01
期刊：
影响因子：
--
通讯作者：
Spiegel, Holger
中科院分区：
文献类型：
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作者：
Schillberg, Stefan;Spiegel, Holger
第一个重组蛋白是在生物反应器中培养的微生物和动物细胞中产生的。这些系统已成为工业规模重组蛋白制造的标准。后来，重组蛋白的生产在整个植物中得到证实，其形态与基于细胞的系统不同，并且需要完全不同的培养条件。随着时间的推移，建立了更多基于植物的生产平台，包括毛状根和细胞悬浮培养物，它们在形态、程序和设备要求方面与传统的基于细胞的系统更加相似。在对该领域的简要概述中，我们解释了为什么基于植物的系统对于生产具有科学和商业应用的有价值的蛋白质越来越有吸引力，但也强调了这些系统必须克服的挑战以实现更广泛的工业利用。我们讨论在植物中生产重组蛋白的各种实验室方案和方法，以及优化产量的策略以及监管和法律框架。
The first recombinant proteins were produced in microbes and animal cells cultivated in bioreactors. These systems have become the standard for industrial-scale recombinant protein manufacturing. Later, the production of recombinant proteins was demonstrated in whole plants, which differ morphologically from cell-based systems and require completely different cultivation conditions. Over time, additional plant-based production platforms were established, including hairy roots and cell suspension cultures, which are more similar to conventional cell-based systems in terms of morphology, procedures, and equipment requirements. In this brief overview of the field, we explain why plant-based systems are becoming increasingly attractive for the production of valuable proteins with scientific and commercial applications, but also highlight the challenges that these systems must overcome to achieve more widespread industrial utilization. We discuss various laboratory protocols and approaches for the production of recombinant proteins in plants, as well as strategies to optimize yields, and the regulatory and legal framework.