Two White Spot Syndrome Virus MicroRNAs Target the Dorsal Gene To Promote Virus Infection in Marsupenaeus japonicus Shrimp
Two White Spot Syndrome Virus MicroRNAs Target the Dorsal Gene To Promote Virus Infection in Marsupenaeus japonicus Shrimp
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两种白斑综合症病毒 MicroRNA 靶向背侧基因促进日本对虾虾的病毒感染
DOI：
10.1128/jvi.02261-16
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发表时间：
2017-04-01
影响因子：
5.4
通讯作者：
Zhang, Xiaobo
中科院分区：
文献类型：
--
作者：
Ren, Qian;Huang, Xin;Zhang, Xiaobo
摘要在真核生物中，微小RNA（miRNAs）是许多生物过程（包括病毒感染）的调节因子。在病毒-宿主相互作用期间，miRNA通常可以靶向不同的基因。然而，在病毒感染过程中，多种miRNA对基因表达的调控尚未得到广泛研究。本研究发现Spaztle（Spz）-Toll-Dorsal-antilipopolysaccharide factor（ALF）信号通路在对虾抗白色斑点综合征病毒（WSSV）入侵的抗病毒免疫中起重要作用。Dorsal是Toll通路中的中心基因，在WSSV感染期间被两种病毒miRNA（WSSV-miR-N13和WSSV-miR-N23）靶向。病毒miRNAs对Dorsal表达的调控抑制了对虾体内Spz-Toll-Dorsal-ALF信号通路，导致病毒感染。我们的研究为病毒-宿主相互作用过程中病毒miRNA介导的Toll信号通路提供了新的见解。在病毒-宿主相互作用过程中，miRNA可以靶向不同的基因。然而，在病毒感染过程中，多种miRNA对基因表达的调控尚未得到广泛研究。本研究结果表明，在白色斑点综合征病毒感染过程中，Toll途径中的中心基因--虾Dorsal基因被两种病毒miRNA靶向。通过病毒miRNAs调控Dorsal表达抑制了对虾体内Spz-Toll-Dorsal-ALF信号通路，导致病毒感染。我们的研究为病毒-宿主相互作用中病毒miRNA介导的Toll信号通路提供了新的见解。
ABSTRACT In eukaryotes, microRNAs (miRNAs) serve as regulators of many biological processes, including virus infection. An miRNA can generally target diverse genes during virus-host interactions. However, the regulation of gene expression by multiple miRNAs has not yet been extensively explored during virus infection. This study found that the Spaztle (Spz)-Toll-Dorsal-antilipopolysaccharide factor (ALF) signaling pathway plays a very important role in antiviral immunity against invasion of white spot syndrome virus (WSSV) in shrimp (Marsupenaeus japonicus). Dorsal, the central gene in the Toll pathway, was targeted by two viral miRNAs (WSSV-miR-N13 and WSSV-miR-N23) during WSSV infection. The regulation of Dorsal expression by viral miRNAs suppressed the Spz-Toll-Dorsal-ALF signaling pathway in shrimp in vivo, leading to virus infection. Our study contributes novel insights into the viral miRNA-mediated Toll signaling pathway during the virus-host interaction. IMPORTANCE An miRNA can target diverse genes during virus-host interactions. However, the regulation of gene expression by multiple miRNAs during virus infection has not yet been extensively explored. The results of this study indicated that the shrimp Dorsal gene, the central gene in the Toll pathway, was targeted by two viral miRNAs during infection with white spot syndrome virus. Regulation of Dorsal expression by viral miRNAs suppressed the Spz-Toll-Dorsal-ALF signaling pathway in shrimp in vivo, leading to virus infection. Our study provides new insight into the viral miRNA-mediated Toll signaling pathway in virus-host interactions.