Early stages in the development of spinal motor neurons.
Early stages in the development of spinal motor neurons.
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脊髓运动神经元发育的早期阶段。
DOI：
10.1002/cne.903200303
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Chiu,AY
中科院分区：
文献类型：
--
作者：
Chen,EW;Chiu,AY
为了确定运动神经元分化的早期事件，通过免疫组织化学在颈脊髓的连续切片上研究了几种发育调节的神经元分子的表达。运动神经元是在胚胎大鼠脊髓内最早出生和分化的神经元之一。它们在胚胎第10天和第11天（E10-11）进行终末有丝分裂，并在E11时获得可检测水平的递质合成酶胆碱乙酰转移酶。5. 68 kD的神经丝蛋白的抗体染色显示运动神经元延伸过程的腹根早在E10。5.针对细胞粘附分子NCAM上两个不同表位的单克隆抗体结合至E10上的肌节。E11开始识别腹角神经元。发育中的神经元，生长相关蛋白，GAP-43，和表面糖蛋白，TAG-1的其他两个标志物，清楚地检测到年轻的运动神经元E11。5.因此，在其前体细胞最终有丝分裂后的36小时内，运动神经元获得细胞骨架、酶和细胞表面成分，这些成分将它们与脊髓内的其他发育细胞区分开来。
In order to identify early events in the differentiation of motor neurons, the expression of several developmentally regulated, neuronal molecules was investigated by immunohistochemistry on consecutive sections of cervical spinal cord. Motor neurons are among the first neurons to be born and to differentiate within the embryonic rat spinal cord. They undergo their terminal mitosis on embryonic days 10 and 11 (E10–11) and acquire detectable levels of the transmitter synthesizing enzyme, choline acetyltransferase, by E11. 5. Staining with antibodies to the 68 kD neurofilament protein revealed motor neurons extending processes out the ventral root as early as E10. 5. Monoclonal antibodies to two different epitopes on the cell adhesive molecule, NCAM, bound to myotomes on E10. 5, and began to recognize ventral horn neurons by E11. Two other markers of developing neurons, the growth-associated protein, GAP-43, and the surface glycoprotein, TAG-1, were clearly detected on young motor neurons by E11. 5. Thus, during the 36 hours following the final mitosis of their precursors, motor neurons acquire cytoskeletal, enzymatic, and cell surface components that distinguish them from other developing cells within the spinal cord.