Porcupine expression is associated with the expression of S100P and other cancer-related molecules in non-small cell lung carcinoma
Porcupine expression is associated with the expression of S100P and other cancer-related molecules in non-small cell lung carcinoma
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DOI：
10.3892/ijo_00000582
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发表时间：
2010-04-01
影响因子：
5.2
通讯作者：
Hofmann, Hans-Stefan
中科院分区：
文献类型：
--
作者：
Bartling, Babett;Rehbein, Grit;Hofmann, Hans-Stefan
o-酰基转移酶Porcupine促进Wnt信号分子的分泌和功能，其刺激各种癌症相关基因的表达。豪猪也参与Wnt诱导的细胞信号转导，通过β-连环蛋白对非小细胞肺癌（NSCLC）细胞。在此，我们报道了Porcupine在人NSCLC组织（n=89）中的表达水平与编码癌症相关分子如β-连环蛋白、缺氧诱导因子-1 α和Jun B的几个基因的表达正相关。然而，与正常肺组织相比，Porcupine的mRNA表达在NSCLC中一般不增加。在NSCLC组织中，我们还发现Porcupine和钙结合蛋白S100 P的表达水平呈正相关，这有助于癌细胞的启动和侵袭。随后的研究表明，DNA低甲基化与5-氮-2 '脱氧胞苷增加了S100 P在NSCLC细胞中的mRNA表达，但不改变豪猪。用小干扰RNA（siRNA）沉默Porcupine的表达降低了NSCLC细胞中S100 P的表达，而用siRNA沉默S100 P的表达不影响Porcupine的表达水平。因此，豪猪碱除了调控DNA甲基化过程外，还可能调控包括S100 P在内的一些肿瘤相关分子的表达。
The o-acyltransferase Porcupine contributes 10 secretion and function of Wnt signaling molecules, which stimulate the expression of various cancer-related genes. Porcupine is also involved on the Wnt-induced cell signaling via beta-Catenin on non-small cell lung carcinoma (NSCLC) cells. Herein, we report that the expression level of Porcupine in human NSCLC tissues (n=89) positively correlates with the expression of several genes coding for cancer-related molecules Such as beta-Catenin, Hypoxia-inducible factor-1 alpha and Jun B. However, the mRNA expression of Porcupine was not generally increased in NSCLC compared to normal lung tissues. In NSCLC tissues we also found a positive correlation between the expression level of Porcupine and the calcium-binding protein S100P, which contributes to initiation and invasion of cancer cells. Subsequent studies showed that the DNA hypomethylation with 5-aza-2'deoxycytidine increased the mRNA expression of S100P in NSCLC cells but did not alter that of Porcupine. Silencing the expression of Porcupine with small interfering (si)RNA reduced the expression of S100P in NSCLC cells, whereas silencing the expression of S100P with siRNA did not effect the level of Porcupine expression. In conclusion, besides DNA methylation processes, Porcupine might regulate the expression of some cancer-related molecules including S100P on human NSCLC.