Detection of G Protein-Coupled Receptor Autoantibodies in Postural Orthostatic Tachycardia Syndrome Using Standard Methodology.
Detection of G Protein-Coupled Receptor Autoantibodies in Postural Orthostatic Tachycardia Syndrome Using Standard Methodology.
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DOI：
10.1161/circulationaha.122.059971
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发表时间：
2022-08-23
期刊：
影响因子：
37.8
通讯作者：
Raj, Satish R.
中科院分区：
文献类型：
--
作者：
Hall, Juliette;Bourne, Kate M.;Vernino, Steven;Hamrefors, Viktor;Kharraziha, Isabella;Nilsson, Jan;Sheldon, Robert S.;Fedorowski, Artur;Raj, Satish R.
体位性心动过速综合征（POTS）是一种主要影响育龄女性的立位耐力障碍。虽然POTS的潜在病理生理学尚未完全了解，但已表明自身免疫可能起作用。本研究的目的是比较POTS患者和健康对照组之间心血管G蛋白偶联受体（GPCR）自身抗体的浓度。从来自加拿大卡尔加里和瑞典马尔默的116名POTS患者（91%女性;年龄29岁）和81名健康对照（84%女性;年龄27岁）中收集血清。使用市售酶联免疫吸附试验（ELISA）评价样本中11种受体（肾上腺素能、毒蕈碱、血管紧张素-II和内皮素）的自身抗体。对照组和POTS患者的自身抗体浓度对所有受体的测试没有显着差异。大多数POTS患者（98.3%）和所有对照（100%）的α 1肾上腺素能受体（α1-AR）自身抗体浓度高于生产商提供的血清阳性阈值（7单位/mL）。对于任何检测的自身抗体，POTS患者与健康对照组中低于诊断阈值的比例没有差异。同样，受试者工作特征曲线显示区分POTS患者和对照组的能力较差。POTS患者和健康对照组在其ELISA衍生的自身抗体浓度方面没有差异。这些发现表明，这些测试是没有用的，以建立自身免疫的作用，在POTS。
Postural orthostatic tachycardia syndrome (POTS) is a disorder of orthostatic intolerance that primarily affects females of child-bearing age. While the underlying pathophysiology of POTS is not fully understood, it has been suggested that autoimmunity may play a role. The aim of this study was to compare concentrations of autoantibodies to cardiovascular G-protein coupled receptors (GPCR) between POTS patients and healthy controls. Sera were collected from 116 POTS patients (91% female; age 29y) and 81 healthy controls (84% female; age 27y) from Calgary, Canada and Malmö, Sweden. Samples were evaluated for autoantibodies to 11 receptors (adrenergic, muscarinic, angiotensin-II, and endothelin) using a commercially available enzyme-linked immunosorbent assay (ELISA). Autoantibody concentrations against all of the receptors tested were not significantly different between controls and POTS patients. The majority of POTS patients (98.3%) and all controls (100%) had alpha-1 adrenergic receptor (α1-AR) autoantibody concentrations above the seropositive threshold provided by the manufacturer (7 units/mL). The proportion of POTS patients versus healthy controls who fell above the diagnostic thresholds were not different for any tested autoantibodies. Similarly, receiver-operating characteristic curves showed a poor ability to discriminate between POTS patients and controls. POTS patients and healthy controls do not differ in their ELISA-derived autoantibody concentrations to cardiovascular GPCRs. These findings suggest that these tests are not useful for establishing the role of autoimmunity in POTS.