Protective effects of ischemic postconditioning compared with gradual reperfusion or preconditioning
Protective effects of ischemic postconditioning compared with gradual reperfusion or preconditioning
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DOI：
10.1002/jnr.21703
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发表时间：
2008-08-15
影响因子：
4.2
通讯作者：
Zhao, Heng
中科院分区：
文献类型：
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作者：
Gao, Xuwen;Ren, Chuancheng;Zhao, Heng
我们研究了后处理的时间因素，评估了逐渐再灌注是否能减少梗死，并将后处理的保护作用与快速和延迟预处理的保护作用进行了比较。通过永久性闭塞大鼠左侧大脑中动脉远端（dMCA）并闭塞双侧颈总动脉（CCA）30 min来产生局灶性缺血。CCA闭塞30 min后，通过反复短暂释放和闭塞CCA进行后处理。通过控制释放双侧CCA产生逐渐再灌注。我们证实，后处理破坏了早期再灌注，但改善脑血流量（CBF）之后。在卒中后2天测量，3个周期（但不是10个周期）的30秒CCA释放和10秒CCA闭塞（30秒/10秒）后处理减少了梗死。此外，10个周期，但不是三个周期，10 s/10 s的后处理减少梗死，但在再灌注后3分钟开始时失去保护。此外，逐渐再灌注也减少了梗死。此外，在卒中前60 min和3天进行的快速和延迟预处理均减少了梗死面积。然而，没有额外的保护检测时，后处理结合快速或延迟预处理。总之，逐渐再灌注减少梗死;后处理的保护依赖于再灌注和闭塞的每个周期的周期数和持续时间以及后处理的开始时间;后处理的保护与快速预处理相当，但不如延迟预处理那么强大。(c)2008 Wiley-Liss，Inc.
We examined the temporal factors of postconditioning, assessed whether gradual reperfusion reduces infarcts, and compared postconditioning's protection with that of both rapid and delayed preconditioning. Focal ischemia was generated by permanent occlusion of the left distal middle cerebral artery (dMCA) combined with 30 min of occlusion of both common carotid arteries (CCA) in rats. Postconditioning was performed by repetitive brief release and occlusion of CCA after 30 min of CCA occlusion. Gradual reperfusion was generated by controlled release of the bilateral CCA. We confirmed that postconditioning disrupted the early reperfusion but improved cerebral blood flow (CBF) thereafter. Postconditioning with three cycles, but not with 10 cycles, of 30 sec CCA release and 10 sec CCA occlusion (30s/10s) reduced infarction measured at 2 days after stroke. In addition, postconditioning with 10 cycles, but not with three cycles, of 10s/10s reduced infarction but it lost protection when initiated at 3 min after reperfusion. In addition, gradual reperfusion also reduced infarction. Moreover, both rapid and delayed preconditioning conducted 60 min and 3 days before stroke reduced infarct sizes. However, no additional protection was detected when postconditioning was combined with either rapid or delayed preconditioning. In conclusion, gradual reperfusion reduced infarction; postconditioning's protection depended on the number of cycles and duration of each cycle of reperfusion and occlusion and the onset time of postconditioning; postconditioning's protection was comparable to that of rapid preconditioning but not as robust as that of delayed preconditioning. (c) 2008 Wiley-Liss, Inc.