RNA recombination plays a major role in genomic change during circulation of coxsackie B viruses
RNA recombination plays a major role in genomic change during circulation of coxsackie B viruses
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DOI：
10.1128/jvi.78.6.2948-2955.2004
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发表时间：
2004-03-01
影响因子：
5.4
通讯作者：
Pallansch, MA
中科院分区：
文献类型：
--
作者：
Oberste, MS;Peñaranda, S;Pallansch, MA
RNA重组已被证明发生在肠道病毒的循环过程中，但大多数研究都集中在脊髓灰质炎病毒。为了研究重组在科萨基B病毒（CVB）进化中的作用，我们测定了1970年至1996年分离的6种CVB血清型中每一种的多个临床分离株的4个基因组间隔的部分序列。测序的区域是5 '-非翻译区（5'-NTR）（350个核苷酸[nt]）、衣壳（VP 4-VP 2，416 nt，和VP 1，类似于320 nt）和聚合酶（3D，491 nt）。使用临床分离株序列和所有65种肠道病毒血清型的原型株序列，为每个基因组区域构建系统发育树。每种CVB血清型的部分VP 1序列相对于血清型是单系的，VP 4-VP 2序列也是单系的，与先前发表的研究一致。然而，在某些情况下，不一致的树形拓扑结构表明，在VP 2和VP 1的测序部分之间发生了型内重组。然而，在衣壳区域之外，相同血清型的分离株不是单系的，表明在5 '-NTR和衣壳之间、衣壳和3D之间或两者之间发生了重组。几乎所有的临床分离株相对于相同血清型的原型菌株是重组的。所有的重组伴侣似乎都是人类肠道病毒B种的成员。这些结果表明，重组是肠道病毒进化过程中的一个频繁事件，但有可能影响重组相容性的遗传限制。
RNA recombination has been shown to occur during circulation of enteroviruses, but most studies have focused on poliovirus. To examine the role of recombination in the evolution of the coxsackie B viruses (CVB), we determined the partial sequences of four genomic intervals for multiple clinical isolates of each of the six CVB serotypes isolated from 1970 to 1996. The regions sequenced were the 5'-nontranslated region (5'-NTR) (350 nucleotides [nt]), capsid (VP4-VP2, 416 nt, and VP1, similar to320 nt), and polymerase (3D, 491 nt). Phylogenetic trees were constructed for each genome region, using the clinical isolate sequences and those of the prototype strains of all 65 enterovirus serotypes. The partial VP1 sequences of each CVB serotype were monophyletic with respect to serotype, as were the VP4-VP2 sequences, in agreement with previously published studies. In some cases, however, incongruent tree topologies suggested that intraserotypic recombination had occurred between the sequenced portions of VP2 and VP1. Outside the capsid region, however, isolates of the same serotype were not monophyletic, indicating that recombination had occurred between the 5'-NTR and capsid, the capsid and 3D, or both. Almost all clinical isolates were recombinant relative to the prototype strain of the same serotype. All of the recombination partners appear to be members of human enterovirus species B. These results suggest that recombination is a frequent event during enterovirus evolution but that there are genetic restrictions that may influence recombinational compatibility.