Effect of the suppression ofBpAP1on the expression of lignin related genes in birch
Effect of the suppression ofBpAP1on the expression of lignin related genes in birch
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抑制BpAP1对桦木木质素相关基因表达的影响
DOI：
10.1007/s11676-020-01232-5
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发表时间：
2020
影响因子：
3
通讯作者：
Jiang Jing
中科院分区：
文献类型：
--
作者：
Huang Haijiao;Wang Shuo;Li Huiyu;Jiang Jing
木质素是植物次生细胞壁的重要组成部分，在维持茎的强度、增强茎的运输能力和提供对多种胁迫的抗性方面起着重要作用。木质素生物合成已成为分子森林生物学研究的热点之一。AP 1转录因子在植物花发育过程中起重要作用。然而，在本研究中，BpAP 1的抑制改变了白色桦树的转录谱，RNA-seq发现BpAP 1的抑制改变了木质素途径相关基因的表达，C4 H/CYP 73 A、POD表达下调，HCT、CCoAOMT、REF 1和CAD表达上调。细胞壁的抑制转基因桦树显着薄于野生型的桦树，和BpAP 1抑制桦树含有较少的木质素。BpAP 1除了调控花的发育外，还可能参与调控白桦木质素生物合成相关基因的表达。本研究为进一步了解木本植物AP 1基因的功能提供了新的思路。
Lignin is an integral part of secondary cell walls in plants and plays important roles in maintaining the strength of stems, enhancing transport ability of stems, and providing resistance to multiple stresses. Lignin biosynthesis has become one of the hotspots in molecular forest biology research. The AP1 transcription factor plays important roles in plant flower development. However, in this study, suppression ofBpAP1altered the transcription profiles of white birch and RNA-seq was used to find that suppression ofBpAP1changed the expression of lignin pathway-related genes;C4H/CYP73A,PODwere down-regulated andHCT,CCoAOMT,REF1andCADwere up-regulated. Cell walls of the suppressed transgenic birch were significantly thinner than the wild type of birch, andBpAP1-repressed birch contained less lignin. In addition to regulation of floral development,BpAP1might play a role in regulating the expression of genes in lignin biosynthesis of birch. This study could provide a new insight into the function ofAP1genes in woody species.