Array comparative genomic hybridization profiling of first-trimester spontaneous abortions that fail to grow in vitro
Array comparative genomic hybridization profiling of first-trimester spontaneous abortions that fail to grow in vitro
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DOI：
10.1002/pd.1230
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发表时间：
2005-10-01
影响因子：
3
通讯作者：
Qumsiyeh, MB
中科院分区：
文献类型：
--
作者：
Benkhalifa, M;Kasakyan, S;Qumsiyeh, MB
目的自然流产标本的细胞遗传学分析因培养失败而受到限制。体外生长失败传统上被怀疑是由于与自然流产（SAB）相关的组织的体内死亡或仅仅是培养中生长的技术因素。（阵列CGH）结果对26例体外培养失败的SAB进行了染色体畸变的比较与已发表的体外培养后细胞遗传学结果的数据。结果显示异常不常见的经典细胞遗传学方法。这些异常包括高比率的双非整倍体和常染色体单体性。所有的数据表明，经典的细胞遗传学的自然流产可能会产生正常核型或选择异常核型，允许细胞增殖在体外，而阵列CGH检测其他abnormal.Conclusion阵列CGH是一个重要的临床检测不平衡的染色体异常细胞是否生长在培养或不分析的情况下，一个或几个细胞。版权所有Q 2005约翰威利父子有限公司。
Objectives Cytogenetic analysis of spontaneous abortion samples can be limited by culture failure. Failure to grow in vitro has traditionally been suspected to be due to in vivo death of tissue associated with spontaneous abortion (SAB) or simply technical factors of growth in culture.Method We used array comparative genomic hybridization (array CGH) to investigate chromosomal imbalances in products of conception that failed to grow in vitro.Results Our data on 26 cases of SABs that failed to grow in culture are compared and contrasted with published data on cytogenetic findings following in vitro culture. The results revealed abnormalities uncommonly seen by classic cytogenetic methods. These abnormalities include high rates of double aneuploidy and autosomal monosomy. The data taken together suggest that classic cytogenetics of spontaneous abortion may yield normal karyotypes or selected abnormal karyotypes that permit cell proliferation in vitro while Array CGH detects other abnormalities.Conclusion Array CGH is becoming an important clinical assay for unbalanced chromosome abnormalities whether cells grow in culture or not and in cases of analysis on one or few cells. Copyright Q 2005 John Wiley & Sons, Ltd.