Macrophage cholesterol removal by triglyceride-phospholipid emulsions.
Macrophage cholesterol removal by triglyceride-phospholipid emulsions.
复制标题
通过甘油三酯-磷脂乳剂去除巨噬细胞胆固醇。
DOI：
10.1016/s0006-291x(88)80552-7
复制
发表时间：
1988
影响因子：
3.1
通讯作者：
Deckelbaum,RJ
中科院分区：
文献类型：
--
作者：
Aviram,M;Williams,KJ;Deckelbaum,RJ
磷脂脂质体以前被证明可以从培养的巨噬细胞泡沫细胞中动员胆固醇。由于Intraperoid是一种临床上可用的磷脂酰胆碱-磷脂乳剂，含有磷脂脂质体和磷脂酰胆碱-乳剂颗粒，因此我们试图研究其对巨噬细胞胆固醇动员的影响。J774巨噬细胞在补充有英脱利匹特的无血清培养基中孵育18小时后，先前负载胆固醇的细胞中的胆固醇酯含量减少了50%，而未负载胆固醇的细胞中的胆固醇酯含量减少了25%。Intraperoid的两种组分（脂质体和乳剂颗粒）均独立引起细胞胆固醇酯减少。我们的结论是，临床上可用的磷脂酰胆碱-磷脂乳剂可以动员巨噬细胞胆固醇在体外。
Phospholipid liposomes were previously shown to mobilize cholesterol from cultured macrophage foam cells. Because Intralipid, a clinically available triglyceride-phospholipid emulsion, contains both phospholipid liposomes and triglyceride-emulsion particles, we sought to study its effect on macrophage cholesterol mobilization. Following an 18h incubation of J774 macrophages in serum-free medium supplemented with Intralipid, cholesteryl ester content decreased by up to 50% in previously cholesterol-loaded cells, and by 25% in non-loaded cells. Both components of Intralipid, liposomes and emulsion particles, independently caused reductions in cellular cholesteryl ester. We conclude that clinically available triglyceride-phospholipid emulsions can mobilize macrophage cholesterol in vitro.