Expression and function of endothelial monocyte-activating polypeptide-II in acute lung inflammation
Expression and function of endothelial monocyte-activating polypeptide-II in acute lung inflammation
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DOI：
10.1007/s00011-006-6162-3
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发表时间：
2007-05-01
影响因子：
6.7
通讯作者：
Singh, B.
中科院分区：
文献类型：
--
作者：
Journeay, W. Shane;Janardhan, K. S.;Singh, B.
目标和设计：我们检验了总内皮单核细胞激活多肽-II（EMAP-II）表达（proEMAP/p43和成熟EMAP-II）在脂多糖（LPS）诱导的急性肺炎症（ALI）中上调，并且成熟EMAP-II在体内诱导单核细胞/巨噬细胞和粒细胞募集。动物气管内滴注250 μ g E.方法：用ELISA法检测EMAP-Ⅱ在大鼠肺组织中的表达，并用光镜和电镜免疫细胞化学方法对EMAP-Ⅱ在1、3和12 h的表达进行定位结果：ELISA显示与对照动物相比，LPS处理的大鼠的肺中总EMAP-II浓度增加（p < 0.05）。与对照组相比，光镜和电镜免疫细胞化学定位总EMAP-II在单核细胞/巨噬细胞和肺泡隔在1和3小时，并在血管平滑肌在12小时后LPS治疗。与对照动物相比，成熟EMAP-II的滴注增加了肺单核细胞/巨噬细胞和粒细胞（p < 0.05）。然而，与LPS处理相比，成熟EMAP-II滴注没有诱导IL-1 β和MIP-2的表达，（p < 0.05）并且引起单核细胞/巨噬细胞的较不剧烈的募集。EMAP-Ⅱ表达在LPS诱导的ALI中增加，并且气管内滴注成熟EMAP-II诱导单核细胞/巨噬细胞和粒细胞募集到肺中，而不刺激IL-1 β或MIP-1。2表情。
Objective and design: We tested the hypothesis that total endothelial monocyte-activating polypeptide-II (EMAP-II) expression (proEMAP/p43 and mature EMAP-II) is up-regulated in lipopolysaccharide (LPS)-induced acute lung inflammation (ALI) and that mature EMAP-II induces monocyte/macrophage and granulocyte recruitment in vivo.Materials: Thirty-five 10 week old, male Sprague-Dawley rats.Treatment: Animals were instilled intratracheally with 250 mu g of E. coli LPS (N = 15) or saline (N = 5) or 20 mu g of mature EMAP-II (N = 5).Methods: Total EMAP-II was quantified using ELISA and the protein was localized with light and electron microscopic immunocytochemistry in lungs of rats at 1, 3 and 12 h (n = 5/group).Results: ELISA showed increased total EMAP-II concentrations (p < 0.05) in lungs from LPS-treated rats compared to control animals. Compared to the controls, light and electron microscopic imunocytochemistry localized total EMAP-II in monocytes/macrophages and alveolar septa at 1 and 3 h and in vascular smooth muscles at 12h post-LPS treatment. Instillation of mature EMAP-II increased lung monocytes/macrophages and granulocytes compared with control animals (p < 0.05). However, compared to the LPS treatment, mature EMAP-II instillation did not induce expression of IL-1 beta and MIP-2 (p < 0.05) and provoked less vigorous recruitment of monocytes/macrophages.Conclusion: EMAP-II expression is increased in LPS-induced ALI, and that intra-tracheal instillation of mature EMAP-II induces recruitment of monocytes/macrophages and granulocytes into the lungs without stimulating IL-1 beta or MIP-2 expression.