Activity density in the open field: a measure for differentiating the effect of psychostimulants
Activity density in the open field: a measure for differentiating the effect of psychostimulants
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DOI：
10.1016/j.pbb.2004.11.004
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发表时间：
2005-02-01
影响因子：
3.6
通讯作者：
Elmer, GI
中科院分区：
文献类型：
--
作者：
Kafkafi, N;Elmer, GI
传统的旷场活动测量无法在小鼠体内提供 D-安非他明 (AMPH) 和可卡因 (COC) 等精神运动兴奋剂之间的明显行为差异。我们使用 Exploration of Exploration (SEE) 软件来研究和开发一种新的行为终点，以表征两种近交系小鼠 C57BL/6 (B6) 和 DBA/2 (D2) 中 AMPH 和 COC 诱导的运动行为的“结构”。我们建议采用一种称为“活动密度”的测量方法来区分 COC 和 AMPH 的行为影响。活动密度定义为活动除以活动发生的范围。它的特征是对行为的限制不仅仅源于不活动。在 B6 和 D2 小鼠中，与产生相同活性水平的 COC 剂量相比，AMPH 通过限制活性范围以剂量依赖性方式增加活性密度。虽然 AMPH 限制了两种基因型的范围，但限制发生的地理区域的特征进一步区分了基因型。因此，新开发的活性密度测量提供了比路径刻板印象更通用的测量，并且可以区分 AMPH 和 COC 在基因型内部和基因型之间的影响。 (C) 2004 Elsevier Inc. 保留所有权利。
Traditional open-field activity measures do not provide a sharp behavioral differentiation across psychomotor stimulants such as d-amphetamine (AMPH) and cocaine (COC) in the mouse. We used Software for the Exploration of Exploration (SEE) to investigate and develop a novel behavioral endpoint to characterize the "structure" of AMPH- and COC-induced locomotor behavior in two inbred strains of mouse, C57BL/6 (B6) and DBA/2 (D2). We suggest a measure we term "activity density" as a means to differentiate the behavioral effects of COC and AMPH. Activity density is defined as the activity divided by the range over which it took place. It characterizes the restriction of behavioral repertoire that does not result merely from inactivity. In both the B6 and D2 mice, AMPH increased activity density in a dose-dependent fashion by restricting the range of activity compared with COC doses producing the same level of activity. While AMPH restricted the range in both genotypes, characterizing the geographical region in which the restriction took place further differentiated the genotypes. The newly developed activity density measure thus provides a more general measure than stereotypy of the path, and can differentiate the effects of AMPH and COC both within and across genotypes. (C) 2004 Elsevier Inc. All rights reserved.