A SURVEY OF THE MICROBIAL COMMUNITY IN THE RHIZOSPHERE OF TWO DOMINANT SHRUBS OF THE NEGEV DESERT HIGHLANDS, ZYGOPHYLLUM DUMOSUM (ZYGOPHYLLACEAE) AND ATRIPLEX HALIMUS (AMARANTHACEAE), USING CULTIVATION-DEPENDENT AND CULTIVATION-INDEPENDENT METHODS
A SURVEY OF THE MICROBIAL COMMUNITY IN THE RHIZOSPHERE OF TWO DOMINANT SHRUBS OF THE NEGEV DESERT HIGHLANDS, ZYGOPHYLLUM DUMOSUM (ZYGOPHYLLACEAE) AND ATRIPLEX HALIMUS (AMARANTHACEAE), USING CULTIVATION-DEPENDENT AND CULTIVATION-INDEPENDENT METHODS
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DOI：
10.3732/ajb.1200615
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发表时间：
2013-09-01
影响因子：
3
通讯作者：
Hirsch, Ann M.
中科院分区：
文献类型：
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作者：
Kaplan, Drora;Maymon, Maskit;Hirsch, Ann M.
研究前提：植物根部占植物生物量的 50% 以上。该生物量的一部分包括根部微生物组，即生活在根部外表面（即根际）附近 1-3 毫米区域内的细菌和真菌的集合。我们假设，生活在以色列内盖夫沙漠恶劣环境的根际和大块土壤中的微生物有潜力用作植物生长促进细菌（PGPB），以提高营养贫乏、干旱土壤中的植物生产力，随着气候变化，这种土壤可能变得更加普遍。方法：我们使用依赖于种植的方法，包括用豆科植物进行陷阱实验来寻找固氮根瘤菌，使用专门的培养基来确定铁螯合铁载体、磷酸盐溶解和纤维素酶活性；不依赖培养的方法，即 16S rDNA 克隆和测序；以及群落水平的生理分析，以发现 2009-2010 年期间与内盖夫沙漠多年生植物霸王和滨藜相关的土壤微生物。 主要结果：我们鉴定了许多 PGPB，包括附生菌和内生菌，它们固氮、螯合铁、溶解磷酸盐和分泌纤维素酶，以及许多其他细菌和一些真菌，从而提供了微生物组的概况支持两种沙漠多年生植物的生长。结论：我们生成了内盖夫沙漠微生物群落的快照，让我们了解其自然状态。与许多干旱环境一样，这片沙漠很容易被用于其他目的，包括太阳能生产和旱地农业。
Premise of the study: Plant roots comprise more than 50% of the plant's biomass. Part of that biomass includes the root microbiome, the assemblage of bacteria and fungi living in the 1-3 mm region adjacent to the external surface of the root, the rhizosphere. We hypothesized that the microorganisms living in the rhizosphere and in bulk soils of the harsh environment of the Negev Desert of Israel had potential for use as plant-growth-promoting bacteria (PGPB) to improve plant productivity in nutrient-poor, arid soils that are likely to become more common as the climate changes.Methods: We used cultivation-dependent methods including trap experiments with legumes to find nitrogen-fixing rhizobia, specialized culture media to determine iron chelation via siderophores and phosphate-solubilizing and cellulase activities; cultivation-independent methods, namely 16S rDNA cloning and sequencing; and also community-level physiological profiling to discover soil microbes associated with the Negev desert perennials Zygophyllum dumosum and Atriplex halimus during the years 2009-2010.Key results: We identified a number of PGPB, both epiphytes and endophytes, which fix nitrogen, chelate iron, solubilize phosphate, and secrete cellulase, as well as many other bacteria and some fungi, thereby providing a profile of the microbiomes that support the growth of two desert perennials.Conclusion: We generated a snapshot of the microbial communities in the Negev Desert, giving us an insight in its natural state. This desert, like many arid environments, is vulnerable to exploitation for other purposes, including solar energy production and dry land farming.