Reversion in vivo after inoculation of a molecular proviral DNA clone of simian immunodeficiency virus with a cytotoxic-T-lymphocyte escape mutation
Reversion in vivo after inoculation of a molecular proviral DNA clone of simian immunodeficiency virus with a cytotoxic-T-lymphocyte escape mutation
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DOI：
10.1128/jvi.79.17.11529-11532.2005
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发表时间：
2005-09-01
影响因子：
5.4
通讯作者：
Matano, T
中科院分区：
文献类型：
--
作者：
Kobayashi, M;Igarashi, H;Matano, T
最近显示了基于疫苗的猴免疫缺陷病毒(SIV)SIVmac239在猕猴体内复制的控制。在对照的过程中，快速选择并控制了一种逃避表位特异性细胞毒T淋巴细胞(CTL)反应的突变病毒。在这项研究中，我们发现在缺乏表位特异性CTL的情况下，野生型病毒在接种CTL逃逸突变体SIV的分子克隆DNA后出现并成为优势病毒。这是第一个描述免疫缺陷病毒CTL逃逸突变的免疫缺陷病毒分子前病毒克隆体内逆转的报道。
Vaccine-based control of the replication of a simian immunodeficiency virus (SIV), SIVmac239, in macaques has recently been shown. In the process of the control, a mutant virus escaping from epitope-specific cytotoxicT-lymphocyte (CTL) responses was rapidly selected and contained. In this study, we show that the wild-type virus appeared and became predominant in the absence of the epitope-specific CTL after inoculation of naive macaques with a molecular clone DNA of the CTL escape mutant SIV. This is the first report describing reversion in vivo from an inoculated, molecular proviral DNA clone of immunodeficiency virus with a CTL escape mutation.