Activation of the ompC gene by the OmpR protein in Escherichia coli. The cis-acting upstream sequence can function in both orientations with respect to the canonical promoter.
Activation of the ompC gene by the OmpR protein in Escherichia coli. The cis-acting upstream sequence can function in both orientations with respect to the canonical promoter.
复制标题
大肠杆菌中 OmpR 蛋白激活 ompC 基因。
DOI：
10.1016/s0021-9258(18)68083-4
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
T. Mizuno
中科院分区：
文献类型：
--
作者：
S. Maeda;T. Mizuno
Expression of the ompC gene coding for an outer membrane protein of Escherichia coli is regulated by a transcriptional activation mechanism that requires the ompR gene product that acts on nucleotide sequences located upstream of the -35 and -10 regions of the ompC promoter. Using an ompC-lacZ fusion gene, the orientation of the cis-acting upstream sequence (OmpR-binding site) with respect to the canonical -35 and -10 regions (RNA polymerase-binding site) was changed. The ompC gene was activated in an OmpR-dependent manner even when the upstream sequence was in the reverse orientation with respect to the -35 and -10 regions, providing that these two DNA elements were aligned stereospecifically on the DNA helix. Evidence is presented that the upstream sequence can increase the transcription efficiency of the ompC promoter in a manner relatively independent of its orientation with respect to the -35 and -10 regions.
