Rice tillering dwarf mutant dwarf3 has increased leaf longevity during darkness-induced senescence or hydrogen peroxide-induced cell death.
Rice tillering dwarf mutant dwarf3 has increased leaf longevity during darkness-induced senescence or hydrogen peroxide-induced cell death.
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DOI：
10.1266/ggs.82.361
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发表时间：
2007-08
影响因子：
1.1
通讯作者：
Haifang Yan;H. Saika;M. Maekawa;I. Takamure;N. Tsutsumi;J. Kyozuka;M. Nakazono
中科院分区：
文献类型：
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作者：
Haifang Yan;H. Saika;M. Maekawa;I. Takamure;N. Tsutsumi;J. Kyozuka;M. Nakazono
已知植物叶片的衰老或细胞死亡可由黑暗或H(2)O(2)诱导。当拟南芥基因MAX2/ORE9被破坏时，叶片衰老或细胞死亡响应上述刺激被延迟。由于水稻（Oryza sativa L.）基因DWARF3 （D3）与MAX2/ORE9同源，我们希望知道D3的破坏是否也会导致叶片寿命的延长。我们发现，与对照系Shiokari相比，d3水稻突变体中黑暗诱导的第三叶衰老或H(2)O(2)诱导的细胞死亡（通过叶绿素降解、膜离子泄漏和衰老相关基因（sag）的表达来测量）延迟了1-3天。此外，拟南芥MAX2/ORE9、MAX3和MAX4的同源基因D3、HTD1和D10的mRNA水平在细胞死亡过程中升高。这些结果表明，水稻中的D3蛋白与拟南芥中的MAX2/ORE9一样，参与了叶片衰老或细胞死亡。
Senescence or cell death in plant leaves is known to be inducible by darkness or H(2)O(2). When the Arabidopsis gene MAX2/ORE9 is disrupted, leaf senescence or cell death in response to the above stimuli is delayed. Because the rice (Oryza sativa L.) gene DWARF3 (D3) is orthologous to MAX2/ORE9, we wished to know whether disruption of D3 also results in increased longevity in leaves. We found that darkness-induced senescence or H(2)O(2)-induced cell death in the third leaf [as measured by chlorophyll degradation, membrane ion leakage and expression of senescence-associated genes (SAGs)] in a d3 rice mutant was delayed by 1-3 d compared to that in its reference line Shiokari. Moreover, the mRNA levels of D3, HTD1 and D10, which are orthologs of Arabidopsis MAX2/ORE9, MAX3 and MAX4, respectively, increased during cell death. These results suggest that D3 protein in rice, like MAX2/ORE9 in Arabidopsis, is involved in leaf senescence or cell death.