Synthetically engineered Medea gene drive system in the worldwide crop pest Drosophila suzukii
Synthetically engineered Medea gene drive system in the worldwide crop pest Drosophila suzukii
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DOI：
10.1073/pnas.1713139115
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发表时间：
2018-05-01
影响因子：
11.1
通讯作者：
Akbari, Omar S.
中科院分区：
文献类型：
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作者：
Buchman, Anna;Marshall, John M.;Akbari, Omar S.
合成基因驱动系统具有巨大的潜力，以取代，改变，或抑制野生种群的重要疾病载体和作物害虫;然而，其效用在不同的人群仍有待证明。在这里，我们报告了一个合成的美狄亚基因驱动系统在一个主要的全球性作物害虫，果蝇Suzukii。我们证明，这种驱动系统，基于一个工程母体“毒素”加上一个连接的胚胎“解毒剂”，是能够偏置孟德尔遗传率高达100%的效率。然而，我们发现，驱力阻力，自然发生的遗传变异和相关的健身成本，可以选择和阻碍这种驱动器的传播。尽管如此，我们的研究结果表明，这种基因驱动可以在野生种群中保持高频率，如果其健身成本或毒素抗性降低，则可以传播到固定，为在这种害虫中开发未来的此类系统提供了明确的道路。
Synthetic gene drive systems possess enormous potential to replace, alter, or suppress wild populations of significant disease vectors and crop pests; however, their utility in diverse populations remains to be demonstrated. Here, we report the creation of a synthetic Medea gene drive system in a major worldwide crop pest, Drosophila suzukii. We demonstrate that this drive system, based on an engineered maternal "toxin" coupled with a linked embryonic "antidote," is capable of biasing Mendelian inheritance rates with up to 100% efficiency. However, we find that drive resistance, resulting from naturally occurring genetic variation and associated fitness costs, can be selected for and hinder the spread of such a drive. Despite this, our results suggest that this gene drive could maintain itself at high frequencies in a wild population and spread to fixation if either its fitness costs or toxin resistance were reduced, providing a clear path forward for developing future such systems in this pest.