Effects of endothelin-1 on calcium and potassium currents in undiseased human ventricular myocytes
Effects of endothelin-1 on calcium and potassium currents in undiseased human ventricular myocytes
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DOI：
10.1007/s004240000400
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发表时间：
2000-11-01
影响因子：
4.5
通讯作者：
Nánási, PP
中科院分区：
文献类型：
--
作者：
Magyar, J;Iost, N;Nánási, PP
内皮素在哺乳动物心肌细胞中具有广泛的电生理作用。这些结果是有争议的，人类数据不可用。本实验观察了内皮素-1（ET-1，8 nmol/l）对离体人心室肌细胞L-型钙电流（ICa-L）和各种钾电流（延迟整流快成分I-Kr、瞬时外向电流I-to和内向整流钾电流I-K1）的影响。通过节段灌注技术使用胶原酶消化从未患病的供体心脏获得细胞。应用膜片钳技术的全细胞配置在37 ℃下测量离子电流。在+5mV时，ET-1使峰值I-Ca，I-L从10.2+/-0.6降至6.8+/-0.8pA/pF（对照组的66.7%，P
Endothelins have been reported to exert a wide range of electrophysiological effects in mammalian cardiac cells. These results are controversial and human data are not available. Our aim was to study the effects of endothelin-l (ET-1, 8 nmol/l) on the L-type calcium current (ICa-L) and various potassium currents (rapid component of the delayed rectifier, I-Kr; transient outward current, I-to; and the inward rectifier K current, I-K1) in isolated human ventricular cardiomyocytes. Cells were obtained from undiseased donor hearts using collagenase digestion via the segment perfusion technique. The whole-cell configuration of the patch-clamp technique was applied to measure ionic currents at 37 degreesC. ET-1 significantly decreased peak I-Ca,I-L from 10.2+/-0.6 to 6.8+/-0.8 pA/pF at +5 mV (66.7% of control, P