The Transcriptional Landscape of the Photosynthetic Model Cyanobacterium Synechocystis sp. PCC6803.
The Transcriptional Landscape of the Photosynthetic Model Cyanobacterium Synechocystis sp. PCC6803.
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DOI：
10.1038/srep22168
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发表时间：
2016-02-29
影响因子：
4.6
通讯作者：
Futschik ME
中科院分区：
文献类型：
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作者：
Hernández-Prieto MA;Semeniuk TA;Giner-Lamia J;Futschik ME
蓝藻通过基因表达的变化表现出适应不同环境条件的强大能力。尽管这种可塑性已在蓝细菌集胞藻模型中得到了广泛研究。 PCC 6803 缺乏对不同条件下协调转录适应的详细分析。在此，我们报告了对 37 项独立研究中进行的 756 项单独微阵列测量的荟萃分析，这是迄今为止对集胞藻转录组最全面的研究。通过严格的统计评估，我们在系统水平上描述了集胞藻基因表达的协调适应。数据评估表明，光合装置的表达变化比其他细胞成分更大。然而，网络分析表明光合作用和各种代谢过程存在显着程度的转录协调，并揭示了光系统 I、II 和藻胆体组分的紧密共同调节。对综合数据的详细检查发现了多种调控模式和新的假定光合基因。有趣的是，全局聚类分析表明代谢基因和调节基因应激对条件的转录反应存在对比。可以通过 CyanoEXpress 网站 (http://cyanoexpress.sysbiolab.eu) 访问集成的集胞藻转录组并进行交互式分析。
Cyanobacteria exhibit a great capacity to adapt to different environmental conditions through changes in gene expression. Although this plasticity has been extensively studied in the model cyanobacterium Synechocystis sp. PCC 6803, a detailed analysis of the coordinated transcriptional adaption across varying conditions is lacking. Here, we report a meta-analysis of 756 individual microarray measurements conducted in 37 independent studies-the most comprehensive study of the Synechocystis transcriptome to date. Using stringent statistical evaluation, we characterized the coordinated adaptation of Synechocystis’ gene expression on systems level. Evaluation of the data revealed that the photosynthetic apparatus is subjected to greater changes in expression than other cellular components. Nevertheless, network analyses indicated a significant degree of transcriptional coordination of photosynthesis and various metabolic processes, and revealed the tight co-regulation of components of photosystems I, II and phycobilisomes. Detailed inspection of the integrated data led to the discovery a variety of regulatory patterns and novel putative photosynthetic genes. Intriguingly, global clustering analyses suggested contrasting transcriptional response of metabolic and regulatory genes stress to conditions. The integrated Synechocystis transcriptome can be accessed and interactively analyzed via the CyanoEXpress website (http://cyanoexpress.sysbiolab.eu).