Isolation and characterization of complementary DNA for N-cym, a gene encoded by the DNA strand opposite to N-myc.
Isolation and characterization of complementary DNA for N-cym, a gene encoded by the DNA strand opposite to N-myc.
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发表时间：
1992-06
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通讯作者：
Barbara C. Armstrong;Geoffrey W. Krystal
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作者：
Barbara C. Armstrong;Geoffrey W. Krystal
N-myc癌基因与许多人类肿瘤的发病机制有关，包括儿童神经母细胞瘤和成人小细胞肺癌。我们已经分离并表征了来自转录单位N-cym的互补DNA克隆，N-cym位于N-myc的相反DNA链上，两个转录单位的5'端之间存在广泛的重叠。N-cym基因可编码109个氨基酸的蛋白质，在胎儿发育期间表达，以及在含有扩增的N-myc基因座的肿瘤细胞系中表达，其中其以非常高的水平表达。虽然存在重叠的、相反链的真核基因的其他实例，但N-myc和N-cym是独特的，因为它们似乎在肿瘤细胞系中在基础生长条件下并响应于分化剂视黄酸而共同调节。这种共调节表明，它们的蛋白质产物在正常发育和肿瘤发生过程中可能是功能相关的。
The N-myc oncogene has been implicated in the pathogenesis of a number of human tumors, including childhood neuroblastoma and adult small cell lung cancer. We have isolated and characterized complementary DNA clones derived from a transcription unit, N-cym, located on the opposite DNA strand to N-myc, with extensive overlap existing between the 5' ends of the two transcription units. The N-cym gene, which can encode a 109-amino acid protein, is expressed during fetal development, as well as in tumor cell lines containing amplified N-myc loci, where it is expressed at very high levels. Although other examples of overlapping, opposite-strand eukaryotic genes exist, N-myc and N-cym are unique in that they appear to be coregulated in tumor cell lines under basal growth conditions and in response to the differentiating agent retinoic acid. This coregulation suggests that their protein products may be functionally interrelated during normal development and oncogenesis.