TUMOR-NECROSIS-FACTOR ALPHA-PRODUCING CELLS IN THE INTESTINAL-MUCOSA OF CHILDREN WITH INFLAMMATORY BOWEL-DISEASE
TUMOR-NECROSIS-FACTOR ALPHA-PRODUCING CELLS IN THE INTESTINAL-MUCOSA OF CHILDREN WITH INFLAMMATORY BOWEL-DISEASE
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DOI：
10.1016/0016-5085(94)90398-0
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发表时间：
1994-06-01
期刊：
影响因子：
29.4
通讯作者：
MACDONALD, TT
中科院分区：
文献类型：
--
作者：
BREESE, EJ;MICHIE, CA;MACDONALD, TT
背景/目的：细胞因子被认为在炎症性肠病(IBD)的组织损伤中起重要作用。方法：采用一种灵敏的反向溶血空斑试验检测克罗恩病、溃疡性结肠炎和非炎症性肠病患儿治疗前后黏膜组织标本中肿瘤坏死因子α分泌细胞的频率。结果：轻度非特异性肠炎患儿活检组织中肿瘤坏死因子α分泌细胞的频率明显高于正常肠黏膜组织标本。与患有非特异性炎症的儿童相比，UC的发病率并没有增加，但克罗恩病的发病率明显高于UC。环孢素A治疗后，肿瘤坏死因子-α分泌细胞频率降低，而激素或肠内营养治疗后，分泌细胞频率无明显变化。结论：炎症肠黏膜中肿瘤坏死因子-α分泌细胞增多，与发病机制无关。在IBD患者中，克罗恩病患者的T细胞水平高于UC，这可能反映了克罗恩病患者T细胞的广泛激活。组织学愈合与分泌肿瘤坏死因子-α细胞的频率无关。
Background/Aims:Cytokines are thought to be important in mediating tissue damage in inflammatory bowel disease (IBD). Many of the in vivo activities of tumor necrosis factor α (TNF-α) match the changes found in IBD, but its importance is controversial.Methods:A sensitive, reverse hemolytic plaque assay was used to determine the frequency of TNF-α secreting cells isolated from mucosal biopsy specimens of children with Crohn's disease or ulcerative colitis (UC) and non-IBD controls before and after medical treatment.Results:Frequency of TNF-α secreting cells was significantly increased in biopsy specimens from children with mild, nonspecific inflammation compared with those with histologically normal intestine. Frequency did not increase in UC compared with children with nonspecific inflammation but was significantly greater in Crohn's disease than in UC. After treatment, the frequency of TNF-α secreting cells was reduced in patients receiving cyclosporin A, not reduced in patients with steroids or enteral nutrition, and not changed with treatment in UC.Conclusions:TNF-α secreting cells are increased in the mucosa of inflamed intestine, regardless of pathogenesis. In patients with IBD, higher levels are seen in Crohn's disease than in UC, probably reflecting the extensive T-cell activation in Crohn's disease. No relation existed between histological healing and the frequency of TNF-α-secreting cells.