Three-dimensional visualization of the Autographa californica multiple nucleopolyhedrovirus occlusion-derived virion envelopment process gives new clues as to its mechanism.
Three-dimensional visualization of the Autographa californica multiple nucleopolyhedrovirus occlusion-derived virion envelopment process gives new clues as to its mechanism.
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DOI：
10.1016/j.virol.2014.11.030
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发表时间：
2015-02
期刊：
影响因子：
3.7
通讯作者：
Yang Shi;Kunpeng Li;Peiping Tang;Yinyin Li;Qiang Zhou;Kai Yang;Qinfen Zhang
中科院分区：
文献类型：
--
作者：
Yang Shi;Kunpeng Li;Peiping Tang;Yinyin Li;Qiang Zhou;Kai Yang;Qinfen Zhang
杆状病毒产生两种病毒体表型，闭合衍生病毒体（ODV）和出芽病毒体（BV）。ODV在细胞核内表达。ODV的形态发生已被广泛研究，然而，微泡形成和ODV在细胞核中的表达的机制仍不清楚。本研究采用电子断层扫描（electron tomography，ET）结合常规电镜技术，对苜蓿银纹夜蛾核型多角体病毒（Autographa californicamultiple nucleopolyhedrovirus，AcMNPV）ODV的体外表达进行了研究。我们的研究结果表明，不仅内，而且外核膜可以内陷和囊泡化成微泡，核内微泡是ODV膜的直接来源。总结了ODV执行过程中的五个主要事件，并据此提出了一个解释ODV执行过程的模型。
Baculoviruses produce two virion phenotypes, occlusion-derived virion (ODV) and budded virion (BV). ODV envelopment occurs in the nucleus. Morphogenesis of the ODV has been studied extensively; however, the mechanisms underlying microvesicle formation and ODV envelopment in nuclei remain unclear. In this study, we used electron tomography (ET) together with the conventional electron microscopy to study the envelopment ofAutographa californicamultiple nucleopolyhedrovirus (AcMNPV) ODV. Our results demonstrate that not only the inner but also the outer nuclear membrane can invaginate and vesiculate into microvesicles and that intranuclear microvesicles are the direct source of the ODV membrane. Five main events in the ODV envelopment process are summarized, from which we propose a model to explain this process.