Controlled recovery of the transcription of nanoparticle-bound DNA by intracellular concentrations of glutathione
Controlled recovery of the transcription of nanoparticle-bound DNA by intracellular concentrations of glutathione
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DOI：
10.1021/bc050173j
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发表时间：
2005-11-01
影响因子：
4.7
通讯作者：
Rotello, VM
中科院分区：
文献类型：
--
作者：
Han, G;Chari, NS;Rotello, VM
带正电荷的三甲基铵功能化混合单层保护簇 (MMPC) 通过互补静电相互作用结合 DNA，从而完全抑制 T7 RNA 聚合酶的 DNA 转录。 DNA 通过细胞内浓度的谷胱甘肽从纳米颗粒中释放出来，从而实现有效的转录。 RNA 产生的恢复是 GSH 剂量依赖性的，通过单层结构的变化可以对释放过程进行相当大的控制。这项工作提出了一种控制 DNA 释放的新方法，在创建转染载体和基因调控系统方面具有潜在的应用前景。
Positively charged trimethylammonium-functionalized mixed monolayer protected clusters (MMPCs) bind DNA through complementary electrostatic interactions, resulting in complete inhibition of DNA transcription of T7 RNA polymerase. DNA was released from the nanoparticle by intracellular concentrations of glutathione, resulting in efficient transcription. The restoration of RNA production was dose-dependent in terms of GSH, with considerable control of the release process possible through variation in monolayer structure. This work presents a new approach to controlled release of DNA, with potential applications in the creation of transfection vectors and gene regulation systems.