Analysis of unintegrated avian RNA tumor virus double-stranded DNA intermediates
Analysis of unintegrated avian RNA tumor virus double-stranded DNA intermediates
复制标题
未整合的禽RNA肿瘤病毒双链DNA中间体的分析
DOI：
10.1128/jvi.28.3.810-818.1978
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发表时间：
1978
影响因子：
5.4
通讯作者：
J. Taylor
中科院分区：
文献类型：
--
作者：
T. Hsu;J. L. Sabran;G. Mark;R. Guntaka;J. Taylor
Guntaka等人先前的研究表明，禽类RNA肿瘤病毒复制的未整合的DNA中间体可以在感染后1天从鹌鹑肿瘤系QT-6的培养物中容易地分离。中间体包括双链线性和共价闭合环状DNA物质。使用Southern的分析程序连同先前获得的关于某些限制性内切酶对禽肉瘤病毒DNA的作用位点的信息，我们进一步表征了病毒DNA中间体。有证据表明，相对于RNA基因组，大多数线性物种具有相当于0.5 × 10（6）+/- 0.3 × 10（6）道尔顿双链DNA的直接末端序列冗余。一些环状形式还具有0.21 X 10（6）+/-0.03 X 10（6）道尔顿的序列冗余度。
Previous studies by Guntaka et al. have shown that the unintegrated DNA intermediates of avian RNA tumor virus replication can be readily isolated from cultures of the quail tumor line QT-6 at 1 day after infection. The intermediates include double-stranded linear and covalently closed circular DNA species. Using the analysis procedure of Southern together with previously obtained information regarding the sites of action of certain restriction endonucleases on avian sarcoma virus DNA, we have further characterized the viral DNA intermediates. Evidence is presented that, relative to the RNA genome, most of the linear species possess a direct terminal sequence redundancy equivalent to 0.5 X 10(6) +/- 0.3 X 10(6) daltons of double-stranded DNA. Some of the circular forms also possess a sequence redundancy of 0.21 X 10(6) +/- 0.03 X 10(6) daltons.