Improving bioconversion of eugenol to coniferyl alcohol by in situ eliminating harmful H2O2.
Improving bioconversion of eugenol to coniferyl alcohol by in situ eliminating harmful H2O2.
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DOI：
10.1016/j.biortech.2018.07.104
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发表时间：
2018-07
影响因子：
11.4
通讯作者：
Yongkun Lv;Xiaozhong Cheng;Ding-quan Wu;G. Du;Jingwen Zhou;Jian Chen
中科院分区：
文献类型：
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作者：
Yongkun Lv;Xiaozhong Cheng;Ding-quan Wu;G. Du;Jingwen Zhou;Jian Chen
松柏醇是一种有价值的化学物质。然而，目前获得松柏醇的方法要么效率低下，要么价格昂贵。单纯青霉香草醇氧化酶（PsVAO）可用于生产松柏醇。然而，PsVAO本质上产生有害的副产物H2O2。利用过氧化氢酶分解h2o2是一种潜在的直接方法；然而，过氧化氢酶也可以表现出过氧化物酶活性，促进松柏醇过度氧化。本研究发现了具有高过氧化氢酶活性和低过氧化物酶活性的过氧化氢酶，并将其引入生物转化系统。结果表明，原位去除h2o2可释放h2o2对PsVAO的抑制作用，提高针叶树醇的产量，消除针叶树醇过度氧化。最终，松柏醇滴度、摩尔得率和产率分别达到22.9 g/L、78.7%和0.5 g/（L × h）。克服了PsVAO固有的缺点，开发了一种高效的针叶醇生产方法。
Coniferyl alcohol is a valuable chemical. However, the current approaches to obtain coniferyl alcohol are either unefficient or expensive.Penicillium simplicissimumvanillyl alcohol oxidase (PsVAO) can be used to produce coniferyl alcohol. However, PsVAO intrinsically produces harmful byproduct H2O2. Utilizing catalase to decompose H2O2is a potential straightforward approach; however, catalase can also exhibit peroxidase activity to facilitate coniferyl alcohol over-oxidation. In this study, catalases exhibiting both high catalase activity and low peroxidase activity were found out, and introduced into the bioconversion systems. Our results showed that eliminating H2O2in situreleased H2O2inhibition of PsVAO, improved coniferyl alcohol production and eliminated coniferyl alcohol over-oxidation. Finally, coniferyl alcohol titer, molar yield, and productivity reached 22.9 g/L, 78.7%, and 0.5 g/(L × h) respectively. An efficient coniferyl alcohol production method was developed by overcoming the intrinsic disadvantages of PsVAO.