Transient receptor potential vanilloid 4-dependent calcium influx and ATP release in mouse and rat gastric epithelia
Transient receptor potential vanilloid 4-dependent calcium influx and ATP release in mouse and rat gastric epithelia
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DOI：
10.3748/wjg.v22.i24.5512
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发表时间：
2016-06-28
影响因子：
4.3
通讯作者：
Sugiyama, Toshiro
中科院分区：
文献类型：
--
作者：
Mihara, Hiroshi;Suzuki, Nobuhiro;Sugiyama, Toshiro
目的：为探讨瞬时受体电位香草酸4（TRPV 4）在小鼠和大鼠胃上皮细胞中的表达及其生理意义，采用RT-PCR和免疫组织化学方法检测TRPV 4在小鼠胃和大鼠正常胃上皮细胞系（RGE 1 -01）中的mRNA和蛋白表达，并采用钙离子成像和电生理方法检测TRPV 4通道活性。ATP的释放通过荧光素酶测定来测量。结果：TRPV 4基因敲除小鼠胃排空功能的改变与WT小鼠胃排空功能的改变有关，TRPV 4基因敲除小鼠胃排空功能的改变与WT小鼠胃排空功能的改变有关。TRPV 4特异性激动剂（GSK 1016790 A）可增加WT小鼠胃上皮细胞和RGE 1 -01细胞的细胞内Ca 2+浓度和/或诱发TRPV 4样电流活性，但对TRPV 4KO细胞无影响。结论：TRPV 4在小鼠和大鼠胃上皮中均有表达，并参与ATP释放和胃排空。
AIM: To explore the expression of transient receptor potential vanilloid 4 (TRPV4) and its physiological meaning in mouse and rat gastric epithelia.METHODS: RT-PCR and immunochemistry were used to detect TRPV4 mRNA and protein expression in mouse stomach and a rat normal gastric epithelial cell line (RGE1-01), while Ca2+-imaging and electrophysiology were used to evaluate TRPV4 channel activity. ATP release was measured by a luciferin-luciferase assay. Gastric emptying was also compared between WT and TRPV4 knockout mice.RESULTS: TRPV4 mRNA and protein were detected in mouse tissues and RGE1-01 cells. A TRPV4-specific agonist (GSK1016790A) increased intracellular Ca2+ concentrations and/or evoked TRPV4-like current activities in WT mouse gastric epithelial cells and RGE1-01 cells, but not TRPV4KO cells. GSK1016790A or mechanical stimuli induced ATP release from RGE1-01 cells while TRPV4 knockout mice displayed delayed gastric emptying in vivo.CONCLUSION: TRPV4 is expressed in mouse and rat gastric epithelium and contributes to ATP release and gastric emptying.