Distal Regeneration Involves the Age Dependent Activity of Branchial Sac Stem Cells in the Ascidian Ciona intestinalis.
Distal Regeneration Involves the Age Dependent Activity of Branchial Sac Stem Cells in the Ascidian Ciona intestinalis.
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DOI：
10.1002/reg2.26
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发表时间：
2015-02-01
期刊：
影响因子：
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通讯作者：
Jeffery, William R
中科院分区：
文献类型：
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作者：
Jeffery, William R
被囊动物具有很高的再生能力，但其潜在的机制及其与生命周期进展的关系还不清楚。在这里，我们调查海鞘被囊玻璃海鞘远端结构的再生。对沿着近端-远端体轴再生潜力的分析表明，远端器官，如虹吸管、它们的色素感觉器官和神经复合体，只能从含有鳃囊的身体碎片中替换。远端再生涉及由在分化之前经历细胞增殖的细胞和在没有细胞增殖的情况下分化的细胞组成的芽基的形成。这两种类型的细胞起源于鳃囊，并出现在芽基在远端损伤后的不同时间。虽然鳃囊干细胞存在于年轻动物中，但它们在失去再生能力的老年动物中耗尽。因此，玻璃海鞘成体含有位于鳃囊中的年龄相关干细胞群，这些干细胞是远端体再生的前体来源。
Tunicates have high capacities for regeneration but the underlying mechanisms and their relationship to life cycle progression are not well understood. Here we investigate the regeneration of distal structures in the ascidian tunicate Ciona intestinalis. Analysis of regenerative potential along the proximal−distal body axis indicated that distal organs, such as the siphons, their pigmented sensory organs, and the neural complex, could only be replaced from body fragments containing the branchial sac. Distal regeneration involves the formation of a blastema composed of cells that undergo cell proliferation prior to differentiation and cells that differentiate without cell proliferation. Both cell types originate in the branchial sac and appear in the blastema at different times after distal injury. Whereas the branchial sac stem cells are present in young animals, they are depleted in old animals that have lost their regeneration capacity. Thus Ciona adults contain a population of age‐related stem cells located in the branchial sac that are a source of precursors for distal body regeneration.