Ultrasmall dopamine-coated nanogolds: preparation, characteristics, and CT imaging.
Ultrasmall dopamine-coated nanogolds: preparation, characteristics, and CT imaging.
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DOI：
10.1080/17458080.2015.1102343
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发表时间：
2016-12-12
影响因子：
2.8
通讯作者：
Wu D
中科院分区：
文献类型：
--
作者：
Yu Y;Wu Y;Liu J;Zhan Y;Wu D
以2-巯基丁二酸为稳定剂，硼氢化钠为还原剂，采用还原法制备了水分散性超小纳米金（WDU AuNPs）及其表面包覆多巴胺的纳米金（WDU AuNPs@DPAs）。这些纳米粒子对计算机断层扫描（CT）成像的影响进行了评价。用马尔文粒度分析仪测量纳米颗粒的粒度分布和Zeta电位，并通过透射电子显微镜观察纳米颗粒的形态。这些特性通过傅里叶变换光谱和紫外/可见光谱得到证实。发现WDU AuNPs@ DPA的尺寸为5.4nm，具有清晰的核-壳结构。3-（4，5-二甲基-2-噻唑基）-2，5-二苯基四氮唑溴化物检测结果表明，WDU AuNPs和WDU AuNPs@ DPA对不同细胞的毒性较低。与碘海醇相比，WDU AuNPs@ DPA显示出更长的循环时间和更大的CT衰减系数，并且可以通过肾脏和膀胱排出。这些纳米粒子显示出相当大的潜力，未来的应用在CT成像。
Water-dispersible ultrasmall nanogolds (WDU AuNPs) and their dopamine-coated nanogolds (WDU AuNPs@DPAs) were prepared by a reduction method with sodium borohydride as a reducing agent and a stabilised agent of 2-mercaptosuccinic acid in aqueous solution. The effects of these nanoparticles on computed tomography (CT) imaging were evaluated. The size distributions and Zeta potential of the nanoparticles were measured with a Malvern size analyser, and nanoparticle morphology was observed by transmission electron microscopy. These characteristics were confirmed by Fourier transform spectroscopy and ultraviolet/visible spectra. It was found that WDU AuNPs@DPAs were 5.4 nm in size with clear core–shell structure. The 3-(4, 5-Dimethyl-2-thiazolyl)-2, 5-diphenyltetrazolium bromide assay results showed that the WDU AuNPs and WDU AuNPs@DPAs were hypotoxic to different cells. The WDU AuNPs@DPAs showed a much longer circulation time and a larger CT attenuation coefficient than iohexol and could be excreted by the kidney and bladder. These nanoparticles showed considerable potential for future application in CT imaging.