Imaging the nanomolar range of nitric oxide with an amplifier-coupled fluorescent indicator in living cells
Imaging the nanomolar range of nitric oxide with an amplifier-coupled fluorescent indicator in living cells
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DOI：
10.1073/pnas.0505136102
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发表时间：
2005-10-11
影响因子：
11.1
通讯作者：
Umezawa, Y
中科院分区：
文献类型：
--
作者：
Sato, M;Hida, N;Umezawa, Y
一氧化氮（NO）是一种小分子的不带电荷的自由基，参与多种生理和病理生理机制。NO由NO合酶的三种亚型产生，即内皮型、神经型和诱导型。当在血管内皮细胞中产生时，NO尤其在血管张力调节中起关键作用。在这里，我们描述了一个荧光素偶联的荧光指示剂NO可视化生理纳摩尔动力学NO在活细胞（检测限为0.1 nM）。这种基因编码的高灵敏度指示剂显示，即使在没有剪切应力的情况下，也会在血管内皮细胞中产生足以舒张血管的约1 nM的NO。基础内皮NO的纳摩尔范围，从而揭示似乎是血管稳态的基础。
Nitric oxide (NO) is a small uncharged free radical that is involved in diverse physiological and pathophysiological mechanisms. NO is generated by three isoforms of NO synthase, endothelial, neuronal, and inducible ones. When generated in vascular endothelial cells, NO plays a key role in vascular tone regulation, in particular. Here, we describe an amplifier-coupled fluorescent indicator for NO to visualize physiological nanomolar dynamics of NO in living cells (detection limit of 0.1 nM). This genetically encoded high-sensitive indicator revealed that approximate to 1 nM of NO, which is enough to relax blood vessels, is generated in vascular endothelial cells even in the absence of shear stress. The nanomolar range of basal endothelial NO thus revealed appears to be fundamental to vascular homeostasis.