Sequence of events in the transformation process in cells infected with a temperature-sensitive transformation mutant of Moloney murine sarcoma virus.
Sequence of events in the transformation process in cells infected with a temperature-sensitive transformation mutant of Moloney murine sarcoma virus.
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莫洛尼鼠肉瘤病毒温度敏感转化突变体感染细胞转化过程中的事件序列。
DOI：
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发表时间：
1981
影响因子：
11.1
通讯作者：
B. Brinkley
中科院分区：
文献类型：
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作者：
R. Brown;J. P. Horn;L. Wible;R. Arlinghaus;B. Brinkley
用莫洛尼鼠肉瘤病毒的温度敏感性转化突变体感染正常大鼠肾细胞，以研究转化过程中发生的生化和形态学变化。感染的细胞在非允许温度（39 ℃）下表现出正常形态，在允许温度（33 ℃）下表现出转化形态。在转变到允许温度后2小时，检测到一种新的病毒蛋白，作为多聚蛋白，估计Mr为85，000（p85）。扫描电子显微镜下的细胞在5小时内转移到允许的温度后，显示他们变得更小，圆形与许多细长的微绒毛。在早期的研究中，发现己糖摄取的变化发生在转变后8-12小时[Horn，J. P.，伍德，T. G.，Blair，D. G. & Arlinghaus，R. B。（1980）Virology 105，516-525]。到48小时，细胞具有完全转化细胞的形态。伴随着形态学的变化是细胞质微管复合物的改变。在非允许的温度下，复合物由微管的花边网络组成。在5小时内，在允许的温度下，花边网络仍然存在，但微管更弥漫染色，更难辨别。到48小时，微管是如此分散，花边网络无法识别。在F-肌动蛋白电缆的变化没有发生，直到24小时后，移动到允许的温度的细胞。去核的细胞在nonpermissive温度和移动的细胞质的允许温度没有导致可检测的p85的合成或在任何改变的细胞质形态或微管复合物，这表明温度敏感的病变影响一些事件发生在细胞核中。
Normal rat kidney cells infected with the temperature-sensitive transformation mutant of Moloney murine sarcoma virus were used to study the biochemical and morphological changes that occur during transformation. The infected cells exhibited a normal morphology at the nonpermissive temperature (39 degrees C) and a transformed morphology at the permissive temperature (33 degrees C). A new viral protein was detected 2 hr after shift to the permissive temperature as a polyprotein with an estimated Mr of 85,000 (p85). Scanning electron microscopy of the cells within 5 hr after shifting them to the permissive temperature showed that they became smaller and rounded with numerous elongated microvilli. In an earlier study, changes in hexose uptake were found to occur 8-12 hr after the shift [Horn, J. P., Wood, T. G., Blair, D. G. & Arlinghaus, R. B. (1980) Virology 105, 516-525]. By 48 hr, the cells had the morphology of a fully transformed cell. Concomitant with the changes in the morphology were alterations in the cytoplasmic microtubule complex. At the nonpermissive temperature, the complex consisted of a lacy network of microtubules. Within 5 hr at the permissive temperature, the lacy network was still present but the microtubules were more diffusely stained and less discernible. By 48 hr, the microtubules were so diffuse that the lacy network could not be recognized. Alterations in the F-actin cables did not occur until 24 hr after shifting the cells to the permissive temperature. Enucleation of the cells at the nonpermissive temperature and shifting the cytoplasts to the permissive temperature did not result in the synthesis of detectable p85 or in any alteration of the cytoplast morphology or microtubule complex, suggesting that the temperature-sensitive lesion affects some event occurring in the nucleus.