In vivo gene transfer of leukemia inhibitory factor (LIF) into mouse endometrium
In vivo gene transfer of leukemia inhibitory factor (LIF) into mouse endometrium
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DOI：
10.1023/a:1014495614792
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发表时间：
2002-02-01
影响因子：
3.1
通讯作者：
Wu, JCH
中科院分区：
文献类型：
--
作者：
Hsieh, YY;Lin, CS;Wu, JCH
目的：白血病抑制因子（LIF）对于胚胎发生和着床很重要。我们的目的是将LIF基因转染到小鼠子宫内膜中。材料和方法：表达质粒携带LIF和荧光素酶基因用于转移。超数排卵后，将 100 只 ICR 小鼠与输精管切除的小鼠交配。然后将 LIF-脂质体（第 1 组）和荧光素酶-脂质体复合物（第 2 组）注射到其子宫腔内（第 0 天）。基因转移后第0-4天通过逆转录聚合酶链式反应检测子宫内膜LIF和荧光素酶表达。以3-磷酸甘油醛脱氢酶(GAPDH)作为内参对基因转染进行标准化。结果：LIF mRNA和荧光素酶活性在第3天达到表达峰值。第1组中，第1-4天LIF/GADPH的比值为0.414.1.096.1.162。和0.782。第2组中，第1-4天的LIF/GADPH分别为0.24、0.22、0.35和0.32。结论：脂质体-DNA混合物可以有效转染小鼠子宫内膜。通过脂质体进行子宫内膜 LIF 转移可能对人类有效。
Purpose: Leukemia inhibitory factor (LIF) is important for embryogenesis and implantation. We aimed to transfect LIF gene into the mouse endometrium.Materials and Methods: Expression plasmids carried LIF and luciferase genes for transfer. After superovulation, 100 ICR mice were mated with vasectomized mice. Then LIF-liposome (Group 1) and luciferase-liposome complexes (Group 2) were injected into their uterine lumen (Day 0). Endometrial LIF and luciferase expressions were detected by reverse transcription-polymerase chain reaction on Days 0-4 post gene transfer. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as an internal control to normalize the gene transfection.Results: LIF mRNA and luciferase activities reached the peak expression on Day 3. In Group 1, the ratios of LIF/GADPH on Days 1-4 were 0.414.1.096.1.162. and 0.782. In Group 2, LIF/GADPH on Days 1-4 were 0.24, 0.22, 0.35, and 0.32.Conclusions: Mouse endometrium could be effectively transfected with liposome-DNA mixtures. Endometrial LIF transfer via liposome may be effective in human.