Phenylethanol promotes adhesion and biofilm formation of the antagonistic yeast Kloeckera apiculata for the control of blue mold on citrus
Phenylethanol promotes adhesion and biofilm formation of the antagonistic yeast Kloeckera apiculata for the control of blue mold on citrus
复制标题
苯乙醇促进拮抗酵母 Kloeckera apiculata 的粘附和生物膜形成，以控制柑橘上的蓝霉
DOI：
10.1111/1567-1364.12139
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发表时间：
2014-06-01
影响因子：
3.2
通讯作者：
Cheng Yunjiang
中科院分区：
文献类型：
--
作者：
Liu Pu;Fang Jingfan;Cheng Yunjiang
酵母Kloeckera apiculata菌株34-9是一株对柑桔采后病害具有生物防治活性的拮抗菌。在先前的研究中，证明了K.apiculata产生芳香醇苯基乙醇。在本研究中，我们发现K.apiculata能够在柑橘类水果上形成生物膜并嵌入细胞外基质，从而在伤口表面和病原体之间形成机械屏障。在马铃薯葡萄糖琼脂培养基中，苯基乙醇作为群体感应分子能促进K.apiculata菌丝的形成，而在柑橘果实中，苯基乙醇处理后，拮抗剂仍保持酵母的形态。它只诱导K.apiculata粘附并形成生物膜。在全基因组计算和实验鉴定与K.apiculata粘附相关的可能基因后，我们鉴定了9个可能参与触发酵母粘附的基因。这些基因中有6个在苯乙醇胁迫处理后被显著诱导。本研究为拮抗酵母K.apiculata防治柑桔青霉病提供了一个新的拮抗菌-病原菌相互作用的生物学模型体系。
The yeast Kloeckera apiculata strain 34-9 is an antagonist with biological control activity against postharvest diseases of citrus fruit. In a previous study it was demonstrated that K.apiculata produced the aromatic alcohol phenylethanol. In the present study, we found that K.apiculata was able to form biofilm on citrus fruit and embed in an extracellular matrix, which created a mechanical barrier interposed between the wound surface and pathogen. As a quorum-sensing molecule, phenylethanol can promote the formation of filaments by K.apiculata in potato dextrose agar medium, whereas on the citrus fruit, the antagonist remains as yeast after being treated with the same concentration of phenylethanol. It only induced K.apiculata to adhere and form biofilm. Following genome-wide computational and experimental identification of the possible genes associated with K.apiculata adhesion, we identified nine genes possibly involved in triggering yeast adhesion. Six of these genes were significantly induced after phenylethanol stress treatment. This study provides a new model system of the biology of the antagonist-pathogen interactions that occur in the antagonistic yeast K.apiculata for the control of blue mold on citrus caused by Penicillium italicum.