Purification of Paracoccus denitrificans cytochrome c552 and sequence analysis of the gene.
Purification of Paracoccus denitrificans cytochrome c552 and sequence analysis of the gene.
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脱氮副球菌细胞色素c552的纯化及基因序列分析。
DOI：
10.1111/j.1432-1033.1995.0259f.x
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
B. Ludwig
中科院分区：
文献类型：
--
作者：
A. Turba;M. Jetzek;B. Ludwig
与线粒体不同，许多细菌在其电子传递系统的不同分支中使用大量的c型细胞色素。在存在于土壤细菌Paracoccus acetificans中的许多细胞色素c中，已经提出膜结合细胞色素（c552）介导细胞色素bc 1复合物和细胞色素-c氧化酶之间的电子传递[Berry，E. A. & Trumpower，B. L.（1985）J.Biol.Chem.260，2458-2467]。我们已经从细胞质膜中纯化了这种细胞色素，并克隆和测序了它的基因cycM。序列分析表明，虽然它的C-末端部分是高度相似的I型细胞色素C，其N-末端部分包含一个疏水性的部分提供膜附着。此外，我们提出了其在呼吸中的功能作用的免疫学证据。
Unlike mitochondria, many bacteria use a large repertoire of c-type cytochromes in different branches of their electron transport system. Among the many cytochromes c present in the soil bacterium Paracoccus denitrificans, a membrane-bound cytochrome (c552) has been suggested to mediate the electron transport between the cytochrome bc1 complex and cytochrome-c oxidase [Berry, E. A. & Trumpower, B. L. (1985) J. Biol. Chem. 260, 2458-2467]. We have purified this cytochrome from cytoplasmic membranes, and cloned and sequenced its gene, cycM. Sequence analysis reveals that, while its C-terminal portion is highly similar to type-I cytochromes c, its N-terminal part contains a hydrophobic segment providing membrane attachment. In addition, we present immunological evidence for its functional role in respiration.