REGIONAL VARIATION IN HDL METABOLISM IN HUMAN FAT-CELLS - EFFECT OF CELL-SIZE
REGIONAL VARIATION IN HDL METABOLISM IN HUMAN FAT-CELLS - EFFECT OF CELL-SIZE
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DOI：
10.1152/ajpendo.1987.252.5.e654
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发表时间：
1987-05-01
影响因子：
--
通讯作者：
ANGEL, A
中科院分区：
文献类型：
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作者：
DESPRES, JP;FONG, BS;ANGEL, A
腹型肥胖与血浆高密度脂蛋白(HDL)胆固醇降低有关，两者都与心血管疾病风险有关。我们观察到，腹部皮下脂肪细胞的质膜比大网膜脂肪细胞质膜具有更大的高密度脂蛋白结合能力。本研究调查了这些结合特征是否可能是由于两个脂肪储存库之间脂肪细胞大小或胆固醇浓度的差异。腹部皮下脂肪和大网膜深部脂肪取自手术中的重度肥胖患者。腹部皮下脂肪细胞明显大于大网膜脂肪细胞，其细胞胆固醇含量明显高于大网膜脂肪细胞。胶原酶分离的脂肪细胞与10ug/ml125I-HDL2或125I-HDL3孵育2小时37℃，研究细胞对高密度脂蛋白的摄取。在这两个仓库中，细胞对125I-HDL2和125I-HDL3的摄取被添加25倍的过量未标记高密度脂蛋白特异性地抑制，并且观察到细胞对125I-HDL2和125I-HDL3的摄取之间存在密切的相关性。在肥胖患者中，皮下细胞对~(125)I-高密度脂蛋白的摄取高于大网膜细胞[5.85±-]。0.53比2.74+-0.30 pmol.cntdo.2小时-1.cntdo.(106个细胞)-1]。细胞摄取~(125)I-高密度脂蛋白与脂肪细胞大小和脂肪细胞胆固醇含量显着相关，但与脂肪细胞胆固醇浓度无关。这些结果表明，肥胖患者皮下细胞与大网膜细胞相比对高密度脂蛋白的摄取是脂肪细胞大小的差异而不是胆固醇浓度(胆固醇与甘油三酯的比率)的差异的结果。高密度脂蛋白与肥大的腹部脂肪细胞的相互作用增加，可能在确定肥胖患者高密度脂蛋白的脂组成中起重要作用。
Abdominal obesity is related to reduced plasma high-density lipoprotein (HDL) cholesterol, and both are associated with cardiovascular disease risk. We have observed that plasma membranes from abdominal subcutaneous adipocytes have a greater HDL binding capacity than omental fat cell plasma membranes. The present study examined whether these binding characteristics could be due to differences in fat cell size or cholesterol concentration between the two adipose depots. Abdominal subcutaneous and deep omental fat were obtained from massively obese patients at surgery. Subcutaneous abdominal fat cells were significantly larger and their cellular cholesterol content greater than omental adipocytes. The uptake of HDL by collagenase-isolated fat cells was studied by incubating the cells for 2 h 37.degree.C with 10 .mu.g/ml 125I-HDL2 or 125I-HDL3. In both depots, the cellular uptake of 125I-HDL2 and 125I-HDL3 was specifically inhibited by addition of 25-fold excess unlabeled HDL and a close correlation was observed between the cellular uptake of 125I-HDL2 and 125I-HDL3. In obese patients, the uptake of 125I-HDL was higher in subcutaneous cells than in omental cells [5.85 .+-. 0.53 vs. 2.74 .+-. 0.30 pmol .cntdot. 2 h-1 .cntdot. (106 cells)-1]. The cellular 125I-HDL uptake was significantly correlated with adipocyte size and fat cell cholesterol content but not with adipocyte cholesterol concentration. These results suggest that the higher HDL uptake observed in subcutaneous cells compared with omental cells in obesity is the results of differences in adipocyte size rather than differences in the cholesterol concentration (cholesterol-to-triglyceride ratio). The increased interaction of HDL with hypertrophied abdominal adipocytes may play an important role in determining the lipid composition of HDL in obesity.